Tensile stress induces α-adaptin C production in mouse calvariae in an organ culture

Possible involvement of endocytosis in mechanical stress-stimulated osteoblast differentiation

Junko Shimomura, Osamu Ishibashi, Mika Ikegame, Tatsuya Yoshizawa, Sadakazu Ejiri, Tadashi Noda, Hiroyuki Kawashima

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We previously demonstrated that tensile stress (TS)-induced osteoblast differentiation eventually led to osteogenesis in an organ culture of mouse calvarial sutures. In the present study, we employed RNA-fingerprinting using an arbitrarily primed polymerase chain reaction (RAP-PCR) to identify α-adaptin C, a component of the endocytosis machinery AP2, as a TS-inducible gene. Protein production, as well as the gene expression of α-adaptin C, was induced by TS as early as 3 h following the initiation of loading. In situ hybridization and immunohistochemical analysis revealed that the induction of α-adaptin C mostly occurred in fibroblastic cells in the sutures, suggesting that it precedes TS-induced osteoblast differentiation. Consistent with this result, TS significantly increased the number of coated pits (CPs) and coated vesicles (CVs) in the undifferentiated fibroblastic cells but not in the osteoblastic cells around calvarial bones. Further, TS-induced osteoblast differentiation was suppressed when endocytosis was inhibited by potassium depletion. These results, taken together, suggest that TS accelerates osteoblast differentiation and osteogenesis, possibly through the induction of the α-adaptin C expression and consequent activation of receptor-mediated endocytosis.

Original languageEnglish
Pages (from-to)488-496
Number of pages9
JournalJournal of Cellular Physiology
Volume195
Issue number3
DOIs
Publication statusPublished - Jun 1 2003
Externally publishedYes

Fingerprint

Mechanical Stress
Organ Culture Techniques
Osteoblasts
Endocytosis
Skull
Tensile stress
Osteogenesis
Sutures
Coated Vesicles
In Situ Hybridization
Potassium
RNA
Gene Expression
Bone and Bones
Polymerase chain reaction
Polymerase Chain Reaction
Gene expression
Machinery
Bone
Genes

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Tensile stress induces α-adaptin C production in mouse calvariae in an organ culture : Possible involvement of endocytosis in mechanical stress-stimulated osteoblast differentiation. / Shimomura, Junko; Ishibashi, Osamu; Ikegame, Mika; Yoshizawa, Tatsuya; Ejiri, Sadakazu; Noda, Tadashi; Kawashima, Hiroyuki.

In: Journal of Cellular Physiology, Vol. 195, No. 3, 01.06.2003, p. 488-496.

Research output: Contribution to journalArticle

Shimomura, Junko ; Ishibashi, Osamu ; Ikegame, Mika ; Yoshizawa, Tatsuya ; Ejiri, Sadakazu ; Noda, Tadashi ; Kawashima, Hiroyuki. / Tensile stress induces α-adaptin C production in mouse calvariae in an organ culture : Possible involvement of endocytosis in mechanical stress-stimulated osteoblast differentiation. In: Journal of Cellular Physiology. 2003 ; Vol. 195, No. 3. pp. 488-496.
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