Temporal profile of CRE DNA-binding activity in the rat hippocampus following a kindling stimulation

Kenichi Kashihara, Keiko Sato, Kazufumi Akiyama, Takeshi Ishihara, Toshiyuki Hayabara, Koji Abe

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

This study was designed to establish a role for cAMP-responsive element (CRE) binding protein (CREB) in signal transduction cascade in the hippocampus associated with kindling. Male Sprague-Dawley rats were kindled from the left amygdala until they exhibited Racine's class 5 generalized seizures [Racine (1972). EMBO J. 11, 3337-3346] Nuclear proteins were extracted from dorsal hippocampi obtained from 0 to 24 h after final kindling stimulation. From these, we evaluated the temporal pattern of CRE DNA-binding activity by use of a gel mobility-shift assay with a 32P-labeled CREB oligonucleotide probe. CRE-binding activity in the hippocampus was enhanced significantly at 2 h and returned to baseline level within 4 h after the stimulation. Our results suggest that CREB may be involved in the hippocampal signal transduction pathway of rats activated in response to a kindling stimulation to the amygdala. However, the transient elevation of CRE-binding activity following a seizure in a kindled animal also suggests that persistent activation of CREB may not be required for maintenance of the kindling phenomenon. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)171-177
Number of pages7
JournalEpilepsy Research
Volume40
Issue number2-3
DOIs
Publication statusPublished - Jul 2000

Fingerprint

Hippocampus
Carrier Proteins
DNA
Amygdala
Signal Transduction
Seizures
Oligonucleotide Probes
Electrophoretic Mobility Shift Assay
Nuclear Proteins
Sprague Dawley Rats
Gels
Maintenance

Keywords

  • CREB
  • Epilepsy
  • Hippocampus
  • Kindling
  • Transcription factor

ASJC Scopus subject areas

  • Clinical Neurology
  • Pediatrics, Perinatology, and Child Health
  • Neurology

Cite this

Temporal profile of CRE DNA-binding activity in the rat hippocampus following a kindling stimulation. / Kashihara, Kenichi; Sato, Keiko; Akiyama, Kazufumi; Ishihara, Takeshi; Hayabara, Toshiyuki; Abe, Koji.

In: Epilepsy Research, Vol. 40, No. 2-3, 07.2000, p. 171-177.

Research output: Contribution to journalArticle

Kashihara, Kenichi ; Sato, Keiko ; Akiyama, Kazufumi ; Ishihara, Takeshi ; Hayabara, Toshiyuki ; Abe, Koji. / Temporal profile of CRE DNA-binding activity in the rat hippocampus following a kindling stimulation. In: Epilepsy Research. 2000 ; Vol. 40, No. 2-3. pp. 171-177.
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