Abstract
This study was designed to establish a role for cAMP-responsive element (CRE) binding protein (CREB) in signal transduction cascade in the hippocampus associated with kindling. Male Sprague-Dawley rats were kindled from the left amygdala until they exhibited Racine's class 5 generalized seizures [Racine (1972). EMBO J. 11, 3337-3346] Nuclear proteins were extracted from dorsal hippocampi obtained from 0 to 24 h after final kindling stimulation. From these, we evaluated the temporal pattern of CRE DNA-binding activity by use of a gel mobility-shift assay with a 32P-labeled CREB oligonucleotide probe. CRE-binding activity in the hippocampus was enhanced significantly at 2 h and returned to baseline level within 4 h after the stimulation. Our results suggest that CREB may be involved in the hippocampal signal transduction pathway of rats activated in response to a kindling stimulation to the amygdala. However, the transient elevation of CRE-binding activity following a seizure in a kindled animal also suggests that persistent activation of CREB may not be required for maintenance of the kindling phenomenon. Copyright (C) 2000 Elsevier Science B.V.
| Original language | English |
|---|---|
| Pages (from-to) | 171-177 |
| Number of pages | 7 |
| Journal | Epilepsy Research |
| Volume | 40 |
| Issue number | 2-3 |
| DOIs | |
| Publication status | Published - Jul 2000 |
Fingerprint
Keywords
- CREB
- Epilepsy
- Hippocampus
- Kindling
- Transcription factor
ASJC Scopus subject areas
- Clinical Neurology
- Pediatrics, Perinatology, and Child Health
- Neurology
Cite this
Temporal profile of CRE DNA-binding activity in the rat hippocampus following a kindling stimulation. / Kashihara, Kenichi; Sato, Keiko; Akiyama, Kazufumi; Ishihara, Takeshi; Hayabara, Toshiyuki; Abe, Koji.
In: Epilepsy Research, Vol. 40, No. 2-3, 07.2000, p. 171-177.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Temporal profile of CRE DNA-binding activity in the rat hippocampus following a kindling stimulation
AU - Kashihara, Kenichi
AU - Sato, Keiko
AU - Akiyama, Kazufumi
AU - Ishihara, Takeshi
AU - Hayabara, Toshiyuki
AU - Abe, Koji
PY - 2000/7
Y1 - 2000/7
N2 - This study was designed to establish a role for cAMP-responsive element (CRE) binding protein (CREB) in signal transduction cascade in the hippocampus associated with kindling. Male Sprague-Dawley rats were kindled from the left amygdala until they exhibited Racine's class 5 generalized seizures [Racine (1972). EMBO J. 11, 3337-3346] Nuclear proteins were extracted from dorsal hippocampi obtained from 0 to 24 h after final kindling stimulation. From these, we evaluated the temporal pattern of CRE DNA-binding activity by use of a gel mobility-shift assay with a 32P-labeled CREB oligonucleotide probe. CRE-binding activity in the hippocampus was enhanced significantly at 2 h and returned to baseline level within 4 h after the stimulation. Our results suggest that CREB may be involved in the hippocampal signal transduction pathway of rats activated in response to a kindling stimulation to the amygdala. However, the transient elevation of CRE-binding activity following a seizure in a kindled animal also suggests that persistent activation of CREB may not be required for maintenance of the kindling phenomenon. Copyright (C) 2000 Elsevier Science B.V.
AB - This study was designed to establish a role for cAMP-responsive element (CRE) binding protein (CREB) in signal transduction cascade in the hippocampus associated with kindling. Male Sprague-Dawley rats were kindled from the left amygdala until they exhibited Racine's class 5 generalized seizures [Racine (1972). EMBO J. 11, 3337-3346] Nuclear proteins were extracted from dorsal hippocampi obtained from 0 to 24 h after final kindling stimulation. From these, we evaluated the temporal pattern of CRE DNA-binding activity by use of a gel mobility-shift assay with a 32P-labeled CREB oligonucleotide probe. CRE-binding activity in the hippocampus was enhanced significantly at 2 h and returned to baseline level within 4 h after the stimulation. Our results suggest that CREB may be involved in the hippocampal signal transduction pathway of rats activated in response to a kindling stimulation to the amygdala. However, the transient elevation of CRE-binding activity following a seizure in a kindled animal also suggests that persistent activation of CREB may not be required for maintenance of the kindling phenomenon. Copyright (C) 2000 Elsevier Science B.V.
KW - CREB
KW - Epilepsy
KW - Hippocampus
KW - Kindling
KW - Transcription factor
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UR - http://www.scopus.com/inward/citedby.url?scp=0034038667&partnerID=8YFLogxK
U2 - 10.1016/S0920-1211(00)00123-6
DO - 10.1016/S0920-1211(00)00123-6
M3 - Article
C2 - 10863144
AN - SCOPUS:0034038667
VL - 40
SP - 171
EP - 177
JO - Epilepsy Research
JF - Epilepsy Research
SN - 0920-1211
IS - 2-3
ER -