Abstract
Numerous studies on oral biofilms have been performed in vitro, although it is difficult to mimic the oral environment. Here we used an in situ model to conduct a quantitative analysis and comprehensive identification of bacterial communities over time by performing deep sequencing of 16S rRNA genes. We show here that the number of viable bacteria in supragingival biofilms increased in two steps. Using scanning and transmission electron microscopy, as well as confocal laser scanning microscopy, we detected gram-positive cocci during the first 8 h. The biofilm was subsequently covered with a thick matrix-like structure composed of different bacterial morphotypes that diversified as the number of bacteria increased. Streptococcus accounted for 420% of the population until 16 h, and obligate anaerobes such as Fusobacterium, Prevotella and Porphyromonas predominated after 48 h, and this increase was statistically significant after 96 h (Po0.05). Together, our data demonstrate that an initial population of facultative anaerobic bacteria was replaced with a population of gram-negative anaerobic bacteria during oral biofilm formation. This study, therefore, contributes to a comprehensive understanding of the composition of the bacterial microbiota involved in the health of the human oral cavity.
| Original language | English |
|---|---|
| Article number | 16018 |
| Journal | npj Biofilms and Microbiomes |
| Volume | 2 |
| DOIs | |
| Publication status | Published - Aug 10 2016 |
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ASJC Scopus subject areas
- Biotechnology
- Microbiology
- Applied Microbiology and Biotechnology
Cite this
Temporal dynamics of bacterial microbiota in the human oral cavity determined using an in situ model of dental biofilms. / Wake, Nanako; Asahi, Yoko; Noiri, Yuichiro; Hayashi, Mikako; Motooka, Daisuke; Nakamura, Shota; Goto, Kazuyoshi; Miura, Jiro; Machi, Hiroyuki; Iida, Tetsuya; Ebisu, Shigeyuki.
In: npj Biofilms and Microbiomes, Vol. 2, 16018, 10.08.2016.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Temporal dynamics of bacterial microbiota in the human oral cavity determined using an in situ model of dental biofilms
AU - Wake, Nanako
AU - Asahi, Yoko
AU - Noiri, Yuichiro
AU - Hayashi, Mikako
AU - Motooka, Daisuke
AU - Nakamura, Shota
AU - Goto, Kazuyoshi
AU - Miura, Jiro
AU - Machi, Hiroyuki
AU - Iida, Tetsuya
AU - Ebisu, Shigeyuki
PY - 2016/8/10
Y1 - 2016/8/10
N2 - Numerous studies on oral biofilms have been performed in vitro, although it is difficult to mimic the oral environment. Here we used an in situ model to conduct a quantitative analysis and comprehensive identification of bacterial communities over time by performing deep sequencing of 16S rRNA genes. We show here that the number of viable bacteria in supragingival biofilms increased in two steps. Using scanning and transmission electron microscopy, as well as confocal laser scanning microscopy, we detected gram-positive cocci during the first 8 h. The biofilm was subsequently covered with a thick matrix-like structure composed of different bacterial morphotypes that diversified as the number of bacteria increased. Streptococcus accounted for 420% of the population until 16 h, and obligate anaerobes such as Fusobacterium, Prevotella and Porphyromonas predominated after 48 h, and this increase was statistically significant after 96 h (Po0.05). Together, our data demonstrate that an initial population of facultative anaerobic bacteria was replaced with a population of gram-negative anaerobic bacteria during oral biofilm formation. This study, therefore, contributes to a comprehensive understanding of the composition of the bacterial microbiota involved in the health of the human oral cavity.
AB - Numerous studies on oral biofilms have been performed in vitro, although it is difficult to mimic the oral environment. Here we used an in situ model to conduct a quantitative analysis and comprehensive identification of bacterial communities over time by performing deep sequencing of 16S rRNA genes. We show here that the number of viable bacteria in supragingival biofilms increased in two steps. Using scanning and transmission electron microscopy, as well as confocal laser scanning microscopy, we detected gram-positive cocci during the first 8 h. The biofilm was subsequently covered with a thick matrix-like structure composed of different bacterial morphotypes that diversified as the number of bacteria increased. Streptococcus accounted for 420% of the population until 16 h, and obligate anaerobes such as Fusobacterium, Prevotella and Porphyromonas predominated after 48 h, and this increase was statistically significant after 96 h (Po0.05). Together, our data demonstrate that an initial population of facultative anaerobic bacteria was replaced with a population of gram-negative anaerobic bacteria during oral biofilm formation. This study, therefore, contributes to a comprehensive understanding of the composition of the bacterial microbiota involved in the health of the human oral cavity.
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U2 - 10.1038/npjbiofilms.2016.18
DO - 10.1038/npjbiofilms.2016.18
M3 - Article
VL - 2
JO - npj Biofilms and Microbiomes
JF - npj Biofilms and Microbiomes
SN - 2055-5008
M1 - 16018
ER -