Temporal dynamics of bacterial microbiota in the human oral cavity determined using an in situ model of dental biofilms

Nanako Wake, Yoko Asahi, Yuichiro Noiri, Mikako Hayashi, Daisuke Motooka, Shota Nakamura, Kazuyoshi Goto, Jiro Miura, Hiroyuki Machi, Tetsuya Iida, Shigeyuki Ebisu

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Numerous studies on oral biofilms have been performed in vitro, although it is difficult to mimic the oral environment. Here we used an in situ model to conduct a quantitative analysis and comprehensive identification of bacterial communities over time by performing deep sequencing of 16S rRNA genes. We show here that the number of viable bacteria in supragingival biofilms increased in two steps. Using scanning and transmission electron microscopy, as well as confocal laser scanning microscopy, we detected gram-positive cocci during the first 8 h. The biofilm was subsequently covered with a thick matrix-like structure composed of different bacterial morphotypes that diversified as the number of bacteria increased. Streptococcus accounted for 420% of the population until 16 h, and obligate anaerobes such as Fusobacterium, Prevotella and Porphyromonas predominated after 48 h, and this increase was statistically significant after 96 h (Po0.05). Together, our data demonstrate that an initial population of facultative anaerobic bacteria was replaced with a population of gram-negative anaerobic bacteria during oral biofilm formation. This study, therefore, contributes to a comprehensive understanding of the composition of the bacterial microbiota involved in the health of the human oral cavity.

Original languageEnglish
Article number16018
Journalnpj Biofilms and Microbiomes
Volume2
DOIs
Publication statusPublished - Aug 10 2016

Fingerprint

Dental Models
Microbiota
Biofilms
Mouth
Gram-Negative Anaerobic Bacteria
Porphyromonas
Fusobacterium
Prevotella
Population
Bacteria
High-Throughput Nucleotide Sequencing
Gram-Positive Cocci
Scanning Transmission Electron Microscopy
Anaerobic Bacteria
Streptococcus
rRNA Genes
Confocal Microscopy
Health

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Temporal dynamics of bacterial microbiota in the human oral cavity determined using an in situ model of dental biofilms. / Wake, Nanako; Asahi, Yoko; Noiri, Yuichiro; Hayashi, Mikako; Motooka, Daisuke; Nakamura, Shota; Goto, Kazuyoshi; Miura, Jiro; Machi, Hiroyuki; Iida, Tetsuya; Ebisu, Shigeyuki.

In: npj Biofilms and Microbiomes, Vol. 2, 16018, 10.08.2016.

Research output: Contribution to journalArticle

Wake, Nanako ; Asahi, Yoko ; Noiri, Yuichiro ; Hayashi, Mikako ; Motooka, Daisuke ; Nakamura, Shota ; Goto, Kazuyoshi ; Miura, Jiro ; Machi, Hiroyuki ; Iida, Tetsuya ; Ebisu, Shigeyuki. / Temporal dynamics of bacterial microbiota in the human oral cavity determined using an in situ model of dental biofilms. In: npj Biofilms and Microbiomes. 2016 ; Vol. 2.
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