Temporal and spatial pattern of expression of the pea phenylalanine ammonia-lyase gene1 promoter in transgenic tobacco

Shinji Kawamata, Koji Shimoharai, Yoshiyuki Imura, Miho Ozaki, Yuki Ichinose, Tomonori Shiraishi, Hitoshi Kunoh, Tetsuji Yamada

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Genes encoding phenylalanine ammonia-lyase (PAL) form a small multigene family with at least three members in pea. Tissue-specific expression of the promoter of a member of PAL gene family (PSPAL1) was investigated in the transgenic tobacco transformants carrying the different modes of chimeric fusion between the PSPAL1 promoter and a bacterial β-glucuronidase (GUS) gene. In stems, at least, strict correlation was found between steady-state levels of Gus-mRNA and enzyme activity. Significantly high level of GUS activity was observed in roots, particularly in meristematic tissues and the pigmented region of petals of transgenic tobacco carrying the translational fusion type B (-1,394 to +140 of PSPAL1 connected to Gus), followed by moderately high level of GUS activity carrying the translational fusion type A (-1,394 to + 117). GUS expression in tissues of mature leaves, however, was very low in these constructs. Extremely low GUS activity was observed in the transformants of transcriptional fusion type (-1,394 to +5), whilst no activity was detected carrying non-transcription fusion type (-1,394 to -27). Furthermore, the pattern of the PSPAL1 expression was characterized in response to pathogen ingress and woundings in transgenic tobacco carrying the translational fusion type B. Woundings itself triggered marked expression of PSPAL1 driven GUS expression at the wounded sites. Inoculation of nonpathogens, Phytophthora capsici, P. boehmeriae and Erisiphe graminis f. sp. hordei, both caused rapid and very clear GUS expression zone along with the development of hypersensitive cell death area where callose was accumulated, however, the inoculation of a pathogen, P. nicotiana caused slow and hazy GUS expression zone along with the lesion development. These results suggest that the expression of pea PSPAL1 promoter is regulated in a similar fashion, at least in a part, in pea and transgenic tobacco, under the plant development and various environmental cues.

Original languageEnglish
Pages (from-to)792-803
Number of pages12
JournalPlant and Cell Physiology
Volume38
Issue number7
Publication statusPublished - Jul 1997

Fingerprint

Phenylalanine Ammonia-Lyase
Glucuronidase
Peas
phenylalanine ammonia-lyase
Tobacco
peas
tobacco
promoter regions
genetically modified organisms
Phytophthora capsici
lesions (plant)
genes
pathogens
callose
Nicotiana
multigene family
corolla
plant development
cell death
enzyme activity

Keywords

  • GUS reporter gene
  • Phenylalanine ammonialyase (EC 4.3.1.5)
  • Phytophthora capsici
  • Phytophthora nicotiana
  • Pisum sativum
  • Promoter activity

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Kawamata, S., Shimoharai, K., Imura, Y., Ozaki, M., Ichinose, Y., Shiraishi, T., ... Yamada, T. (1997). Temporal and spatial pattern of expression of the pea phenylalanine ammonia-lyase gene1 promoter in transgenic tobacco. Plant and Cell Physiology, 38(7), 792-803.

Temporal and spatial pattern of expression of the pea phenylalanine ammonia-lyase gene1 promoter in transgenic tobacco. / Kawamata, Shinji; Shimoharai, Koji; Imura, Yoshiyuki; Ozaki, Miho; Ichinose, Yuki; Shiraishi, Tomonori; Kunoh, Hitoshi; Yamada, Tetsuji.

In: Plant and Cell Physiology, Vol. 38, No. 7, 07.1997, p. 792-803.

Research output: Contribution to journalArticle

Kawamata, S, Shimoharai, K, Imura, Y, Ozaki, M, Ichinose, Y, Shiraishi, T, Kunoh, H & Yamada, T 1997, 'Temporal and spatial pattern of expression of the pea phenylalanine ammonia-lyase gene1 promoter in transgenic tobacco', Plant and Cell Physiology, vol. 38, no. 7, pp. 792-803.
Kawamata, Shinji ; Shimoharai, Koji ; Imura, Yoshiyuki ; Ozaki, Miho ; Ichinose, Yuki ; Shiraishi, Tomonori ; Kunoh, Hitoshi ; Yamada, Tetsuji. / Temporal and spatial pattern of expression of the pea phenylalanine ammonia-lyase gene1 promoter in transgenic tobacco. In: Plant and Cell Physiology. 1997 ; Vol. 38, No. 7. pp. 792-803.
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