Temperature-sensitive defects of the GSP1gene, yeast Ran homologue, activate the Tel1-dependent pathway

Naoyuki Hayashi, Seishi Murakami, Susumu Tsurusaki, Zen ichiro Nagaura, Masaya Oki, Hideo Nishitani, Masahiko Kobayashi, Hiroko Shimizu, Ken-ichi Yamamoto, Takeharu Nishimoto

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

RanGTPase is involved in many cellular processes. It functions in nuclear-cytosolic transport and centrosome formation. Ran also localizes to chromatin as RCC1 does, its guanine nucleotide exchange factor, but Ran's function on chromatin is not known. We found that gsp1, a temperature-sensitive mutant of GSP1, a Saccharomyces cerevisiae Ran homologue, suppressed the hydroxyurea (HU) and ultra violet (UV) sensitivities of the mec1 mutant. In UV-irradiated mec1 gsp1 cells, Rad53 was phosphorylated despite the lack of Mec1. This suppression depended on the TEL1 gene, given that the triple mutant, mec1 gsp1 tel1, was unable to grow. The gsp1 mutations also suppressed the HU sensitivity of the rad9 mutant in a Tel1-dependent manner, but not the HU sensitivity of the rad53 mutant. These results indicated that Rad53 was activated by the Tel1 pathway in mec1 gsp1 cells, suggesting that Gsp1 helps regulate the role switching the ATM family kinases Mec1 and Tel1.

Original languageEnglish
Pages (from-to)330-336
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume353
Issue number2
DOIs
Publication statusPublished - Feb 9 2007
Externally publishedYes

Fingerprint

Hydroxyurea
Yeast
Yeasts
Defects
Temperature
Chromatin
Guanine Nucleotide Exchange Factors
Centrosome
Cell Nucleus Active Transport
Automatic teller machines
Saccharomyces cerevisiae
Phosphotransferases
Genes
Mutation

Keywords

  • ATM kinase
  • Checkpoint
  • Gsp1
  • Mec1
  • Rad53
  • Ran
  • Replication
  • Signal transduction
  • Tel1
  • Yeast

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Temperature-sensitive defects of the GSP1gene, yeast Ran homologue, activate the Tel1-dependent pathway. / Hayashi, Naoyuki; Murakami, Seishi; Tsurusaki, Susumu; Nagaura, Zen ichiro; Oki, Masaya; Nishitani, Hideo; Kobayashi, Masahiko; Shimizu, Hiroko; Yamamoto, Ken-ichi; Nishimoto, Takeharu.

In: Biochemical and Biophysical Research Communications, Vol. 353, No. 2, 09.02.2007, p. 330-336.

Research output: Contribution to journalArticle

Hayashi, N, Murakami, S, Tsurusaki, S, Nagaura, ZI, Oki, M, Nishitani, H, Kobayashi, M, Shimizu, H, Yamamoto, K & Nishimoto, T 2007, 'Temperature-sensitive defects of the GSP1gene, yeast Ran homologue, activate the Tel1-dependent pathway', Biochemical and Biophysical Research Communications, vol. 353, no. 2, pp. 330-336. https://doi.org/10.1016/j.bbrc.2006.12.028
Hayashi, Naoyuki ; Murakami, Seishi ; Tsurusaki, Susumu ; Nagaura, Zen ichiro ; Oki, Masaya ; Nishitani, Hideo ; Kobayashi, Masahiko ; Shimizu, Hiroko ; Yamamoto, Ken-ichi ; Nishimoto, Takeharu. / Temperature-sensitive defects of the GSP1gene, yeast Ran homologue, activate the Tel1-dependent pathway. In: Biochemical and Biophysical Research Communications. 2007 ; Vol. 353, No. 2. pp. 330-336.
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