Temperature entrainment of the circadian cuticle deposition rhythm in drosophila melanogaster

Chihiro Ito, Shin G. Goto, Kenji Tomioka, Hideharu Numata

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Abstract

The cuticle deposition rhythm, which is observed in the apodeme of the furca in the thorax, is controlled by a peripheral circadian clock in the epidermal cells and entrained to light-dark (LD) cycles via CRYPTOCHROME (CRY) in Drosophila melanogaster. In the present study, we examined the effects of temperature (TC) cycles and the combination of LD and TC cycles on entrainment of the cuticle deposition rhythm. The rhythm was entrained to TC cycles, whose period was 28 h. In T = 21 and 24 h, the rhythm was entrained to TC cycles in some individuals. CRY is not necessary for temperature entrainment of the cuticle deposition rhythm because the rhythm in cryb (lacking functional CRY) was entrained to TC cycles. Temperature entrainment of the rhythm was achieved even when the thoraxes or furcae were cultured in vitro, suggesting that the mechanism for temperature entrainment is independent of the central clock in the brain and the site of the thermoreception resides in the epidermal cells. When LD and TC cycles with different periods were applied, the rhythm was entrained to LD cycles with a slight influence of TC cycles. Thus, the LD cycle is a stronger zeitgeber than the TC cycle. The variance of the number of the cuticle layers decreased in the flies kept under LD and TC cycles with the same period in which the thermophase coincided with the photophase. Therefore, we conclude that LD and TC cycles synergistically entrain the rhythm. Synergistic effects of LD and TC cycles on entrainment were also observed even when the thoraxes were cultured in vitro, suggesting that the light and temperature information is integrated within the peripheral circadian system.

Original languageEnglish
Pages (from-to)14-23
Number of pages10
JournalJournal of biological rhythms
Volume26
Issue number1
DOIs
Publication statusPublished - Feb 1 2011

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Keywords

  • CRYPTOCHROME
  • circadian rhythm
  • cuticle deposition
  • peripheral clock
  • phospholipase C
  • temperature cycle
  • temperature-induced oscillation

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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