Temperature dependent LH1 → RC energy transfer in purple bacteria Tch. tepidum with shiftable LH1-Qy band: A natural system to investigate thermally activated energy transfer in photosynthesis

Fei Ma, Long-Jiang Yu, Zheng Yu Wang-Otomo, Rienk Van Grondelle

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The native LH1-RC complex of the purple bacterium Thermochromatium (Tch.) tepidum has an ultra-red LH1-Qy absorption at 915 nm, which can shift to 893 and 882 nm by means of chemical modifications. These unique complexes are a good natural system to investigate the thermally activated energy transfer process, with the donor energies different while the other factors (such as the acceptor energy, special pair at 890 nm, and the distance/relative orientation between the donor and acceptor) remain the same. The native B915-RC, B893-RC and B882-RC complexes, as well as the LH1-RC complex of Rhodobacter (Rba.) sphaeroides were studied by temperature-dependent time-resolved absorption spectroscopy. The energy transfer time constants, kET - 1, are 65, 45, 46 and 45 ps at room temperature while 225, 58, 85, 33 ps at 77 K for the B915-RC, B893-RC, B882-RC and Rba. sphaeroides LH1-RC, respectively. The dependences of kET on temperature have different trends. The reorganization energies are determined to be 70, 290, 200 and 45 cm- 1, respectively, by fitting kET vs temperature using Marcus equation. The activation energies are 200, 60, 115 and 20 cm- 1, respectively. The influences of the structure (the arrangement of the 32 BChl a molecules) on kET are discussed based on these results, to reveal how the B915-RC complex accomplishes its energy transfer function with a large uphill energy of 290 cm- 1.

Original languageEnglish
Pages (from-to)408-414
Number of pages7
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1857
Issue number4
DOIs
Publication statusPublished - Apr 1 2016
Externally publishedYes

Fingerprint

Proteobacteria
Photosynthesis
Energy Transfer
Energy transfer
Rhodobacter sphaeroides
Temperature
Chemical modification
Absorption spectroscopy
Transfer functions
Spectrum Analysis
Activation energy
Molecules

Keywords

  • LH1 → RC energy transfer
  • Thermally activated energy transfer
  • Thermochromatium (Tch.) tepidum
  • Time-resolved absorption spectroscopy

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology

Cite this

Temperature dependent LH1 → RC energy transfer in purple bacteria Tch. tepidum with shiftable LH1-Qy band : A natural system to investigate thermally activated energy transfer in photosynthesis. / Ma, Fei; Yu, Long-Jiang; Wang-Otomo, Zheng Yu; Van Grondelle, Rienk.

In: Biochimica et Biophysica Acta - Bioenergetics, Vol. 1857, No. 4, 01.04.2016, p. 408-414.

Research output: Contribution to journalArticle

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abstract = "The native LH1-RC complex of the purple bacterium Thermochromatium (Tch.) tepidum has an ultra-red LH1-Qy absorption at 915 nm, which can shift to 893 and 882 nm by means of chemical modifications. These unique complexes are a good natural system to investigate the thermally activated energy transfer process, with the donor energies different while the other factors (such as the acceptor energy, special pair at 890 nm, and the distance/relative orientation between the donor and acceptor) remain the same. The native B915-RC, B893-RC and B882-RC complexes, as well as the LH1-RC complex of Rhodobacter (Rba.) sphaeroides were studied by temperature-dependent time-resolved absorption spectroscopy. The energy transfer time constants, kET - 1, are 65, 45, 46 and 45 ps at room temperature while 225, 58, 85, 33 ps at 77 K for the B915-RC, B893-RC, B882-RC and Rba. sphaeroides LH1-RC, respectively. The dependences of kET on temperature have different trends. The reorganization energies are determined to be 70, 290, 200 and 45 cm- 1, respectively, by fitting kET vs temperature using Marcus equation. The activation energies are 200, 60, 115 and 20 cm- 1, respectively. The influences of the structure (the arrangement of the 32 BChl a molecules) on kET are discussed based on these results, to reveal how the B915-RC complex accomplishes its energy transfer function with a large uphill energy of 290 cm- 1.",
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