TY - JOUR
T1 - Temperature-dependent fasciation mutants provide a link between mitochondrial RNA processing and lateral root morphogenesis
AU - Otsuka, Kurataka
AU - Mamiya, Akihito
AU - Konishi, Mineko
AU - Nozaki, Mamoru
AU - Kinoshita, Atsuko
AU - Tamaki, Hiroaki
AU - Arita, Masaki
AU - Saito, Masato
AU - Yamamoto, Kayoko
AU - Hachiya, Takushi
AU - Noguchi, Ko
AU - Ueda, Takashi
AU - Yagi, Yusuke
AU - Kobayashi, Takehito
AU - Nakamura, Takahiro
AU - Sato, Yasushi
AU - Hirayama, Takashi
AU - Sugiyama, Munetaka
N1 - Publisher Copyright:
© Otsuka et al.
PY - 2021
Y1 - 2021
N2 - Although mechanisms that activate organogenesis in plants are well established, much less is known about the subsequent fine-tuning of cell proliferation, which is crucial for creating properly structured and sized organs. Here we show, through analysis of temperature-dependent fasciation (TDF) mutants of Arabidopsis, root redifferentiation defective 1 (rrd1), rrd2, and root initiation defective 4 (rid4), that mitochondrial RNA processing is required for limiting cell division during early lateral root (LR) organogenesis. These mutants formed abnormally broadened (i.e. fasciated) LRs under high-temperature conditions due to extra cell division. All TDF proteins localized to mitochondria, where they were found to participate in RNA processing: RRD1 in mRNA deadenylation, and RRD2 and RID4 in mRNA editing. Further analysis suggested that LR fasciation in the TDF mutants is triggered by reactive oxygen species generation caused by defective mitochondrial respiration. Our findings provide novel clues for the physiological significance of mitochondrial activities in plant organogenesis.
AB - Although mechanisms that activate organogenesis in plants are well established, much less is known about the subsequent fine-tuning of cell proliferation, which is crucial for creating properly structured and sized organs. Here we show, through analysis of temperature-dependent fasciation (TDF) mutants of Arabidopsis, root redifferentiation defective 1 (rrd1), rrd2, and root initiation defective 4 (rid4), that mitochondrial RNA processing is required for limiting cell division during early lateral root (LR) organogenesis. These mutants formed abnormally broadened (i.e. fasciated) LRs under high-temperature conditions due to extra cell division. All TDF proteins localized to mitochondria, where they were found to participate in RNA processing: RRD1 in mRNA deadenylation, and RRD2 and RID4 in mRNA editing. Further analysis suggested that LR fasciation in the TDF mutants is triggered by reactive oxygen species generation caused by defective mitochondrial respiration. Our findings provide novel clues for the physiological significance of mitochondrial activities in plant organogenesis.
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U2 - 10.7554/eLife.61611
DO - 10.7554/eLife.61611
M3 - Article
C2 - 33443014
AN - SCOPUS:85100062891
VL - 10
SP - 1
EP - 26
JO - eLife
JF - eLife
SN - 2050-084X
M1 - e61611
ER -