TEM characterization of a silorane composite bonded to enamel/dentin

Atsushi Mine, Jan De Munck, Annelies Van Ende, Marcio Vivan Cardoso, Takuo Kuboki, Yasuhiro Yoshida, Bart Van Meerbeek

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Objectives: The low-shrinking composite composed of combined siloxane-oxirane technology (Filtek Silorane, 3M ESPE, Seefeld, Germany) required the development of a specific adhesive (Silorane System Adhesive, 3M ESPE), in particular because of the high hydrophobicity of the silorane composite. The purpose of this study was to characterize the interfacial ultra-structure at enamel and dentin using transmission electron microscopy (TEM). Methods: Non-demineralized/demineralized 70-90 nm sections were prepared following common TEM specimen processing procedures. Results: TEM revealed a typical twofold build-up of the adhesive resin, resulting in a total adhesive layer thickness of 10-20 μm. At bur-cut enamel, a tight interface without distinct dissolution of hydroxyapatite was observed. At bur-cut dentin, a relatively thin hybrid layer of maximum a few hundreds of nanometer was formed without clear surface demineralization. No clear resin tags were formed. At fractured dentin, the interaction appeared very superficial (100-200 nm). Distinct resin tags were formed due to the absence of smear plugs. Silver-nitrate infiltration showed a varying pattern of both spot- and cluster-like appearance of nano-leakage. Traces of Ag were typically detected along some part of the enamel-adhesive interface and/or between the two adhesive resin layers. Substantially more Ag-infiltration was observed along the dentin-adhesive interface of bur-cut dentin, as compared to that of fractured dentin. Conclusions: The nano-interaction of Silorane System Adhesive should be attributed to its relatively high pH of 2.7. The obtained tight interface at both enamel and dentin indicates that the two-step self-etch adhesive effectively bridged the hydrophilic tooth substrate with the hydrophobic silorane composite.

Original languageEnglish
Pages (from-to)524-532
Number of pages9
JournalDental Materials
Volume26
Issue number6
DOIs
Publication statusPublished - Jun 2010

Fingerprint

Silorane Resins
Enamels
Dentin
Dental Enamel
Transmission Electron Microscopy
Adhesives
Transmission electron microscopy
Composite materials
Resins
Infiltration
Siloxanes
Silver Nitrate
Ethylene Oxide
Durapatite
Hydrophobicity
Hydrophobic and Hydrophilic Interactions
Hydroxyapatite
Germany
Nitrates
Tooth

Keywords

  • Adhesion
  • Low-shrinking composite
  • Nano-interaction
  • Self-etch adhesive
  • Silorane

ASJC Scopus subject areas

  • Dentistry(all)
  • Materials Science(all)
  • Mechanics of Materials
  • Medicine(all)

Cite this

Mine, A., De Munck, J., Van Ende, A., Cardoso, M. V., Kuboki, T., Yoshida, Y., & Van Meerbeek, B. (2010). TEM characterization of a silorane composite bonded to enamel/dentin. Dental Materials, 26(6), 524-532. https://doi.org/10.1016/j.dental.2010.01.010

TEM characterization of a silorane composite bonded to enamel/dentin. / Mine, Atsushi; De Munck, Jan; Van Ende, Annelies; Cardoso, Marcio Vivan; Kuboki, Takuo; Yoshida, Yasuhiro; Van Meerbeek, Bart.

In: Dental Materials, Vol. 26, No. 6, 06.2010, p. 524-532.

Research output: Contribution to journalArticle

Mine, A, De Munck, J, Van Ende, A, Cardoso, MV, Kuboki, T, Yoshida, Y & Van Meerbeek, B 2010, 'TEM characterization of a silorane composite bonded to enamel/dentin', Dental Materials, vol. 26, no. 6, pp. 524-532. https://doi.org/10.1016/j.dental.2010.01.010
Mine, Atsushi ; De Munck, Jan ; Van Ende, Annelies ; Cardoso, Marcio Vivan ; Kuboki, Takuo ; Yoshida, Yasuhiro ; Van Meerbeek, Bart. / TEM characterization of a silorane composite bonded to enamel/dentin. In: Dental Materials. 2010 ; Vol. 26, No. 6. pp. 524-532.
@article{d2605ac89631480baf8d5eafa5304a98,
title = "TEM characterization of a silorane composite bonded to enamel/dentin",
abstract = "Objectives: The low-shrinking composite composed of combined siloxane-oxirane technology (Filtek Silorane, 3M ESPE, Seefeld, Germany) required the development of a specific adhesive (Silorane System Adhesive, 3M ESPE), in particular because of the high hydrophobicity of the silorane composite. The purpose of this study was to characterize the interfacial ultra-structure at enamel and dentin using transmission electron microscopy (TEM). Methods: Non-demineralized/demineralized 70-90 nm sections were prepared following common TEM specimen processing procedures. Results: TEM revealed a typical twofold build-up of the adhesive resin, resulting in a total adhesive layer thickness of 10-20 μm. At bur-cut enamel, a tight interface without distinct dissolution of hydroxyapatite was observed. At bur-cut dentin, a relatively thin hybrid layer of maximum a few hundreds of nanometer was formed without clear surface demineralization. No clear resin tags were formed. At fractured dentin, the interaction appeared very superficial (100-200 nm). Distinct resin tags were formed due to the absence of smear plugs. Silver-nitrate infiltration showed a varying pattern of both spot- and cluster-like appearance of nano-leakage. Traces of Ag were typically detected along some part of the enamel-adhesive interface and/or between the two adhesive resin layers. Substantially more Ag-infiltration was observed along the dentin-adhesive interface of bur-cut dentin, as compared to that of fractured dentin. Conclusions: The nano-interaction of Silorane System Adhesive should be attributed to its relatively high pH of 2.7. The obtained tight interface at both enamel and dentin indicates that the two-step self-etch adhesive effectively bridged the hydrophilic tooth substrate with the hydrophobic silorane composite.",
keywords = "Adhesion, Low-shrinking composite, Nano-interaction, Self-etch adhesive, Silorane",
author = "Atsushi Mine and {De Munck}, Jan and {Van Ende}, Annelies and Cardoso, {Marcio Vivan} and Takuo Kuboki and Yasuhiro Yoshida and {Van Meerbeek}, Bart",
year = "2010",
month = "6",
doi = "10.1016/j.dental.2010.01.010",
language = "English",
volume = "26",
pages = "524--532",
journal = "Dental Materials",
issn = "0109-5641",
publisher = "Elsevier Science",
number = "6",

}

TY - JOUR

T1 - TEM characterization of a silorane composite bonded to enamel/dentin

AU - Mine, Atsushi

AU - De Munck, Jan

AU - Van Ende, Annelies

AU - Cardoso, Marcio Vivan

AU - Kuboki, Takuo

AU - Yoshida, Yasuhiro

AU - Van Meerbeek, Bart

PY - 2010/6

Y1 - 2010/6

N2 - Objectives: The low-shrinking composite composed of combined siloxane-oxirane technology (Filtek Silorane, 3M ESPE, Seefeld, Germany) required the development of a specific adhesive (Silorane System Adhesive, 3M ESPE), in particular because of the high hydrophobicity of the silorane composite. The purpose of this study was to characterize the interfacial ultra-structure at enamel and dentin using transmission electron microscopy (TEM). Methods: Non-demineralized/demineralized 70-90 nm sections were prepared following common TEM specimen processing procedures. Results: TEM revealed a typical twofold build-up of the adhesive resin, resulting in a total adhesive layer thickness of 10-20 μm. At bur-cut enamel, a tight interface without distinct dissolution of hydroxyapatite was observed. At bur-cut dentin, a relatively thin hybrid layer of maximum a few hundreds of nanometer was formed without clear surface demineralization. No clear resin tags were formed. At fractured dentin, the interaction appeared very superficial (100-200 nm). Distinct resin tags were formed due to the absence of smear plugs. Silver-nitrate infiltration showed a varying pattern of both spot- and cluster-like appearance of nano-leakage. Traces of Ag were typically detected along some part of the enamel-adhesive interface and/or between the two adhesive resin layers. Substantially more Ag-infiltration was observed along the dentin-adhesive interface of bur-cut dentin, as compared to that of fractured dentin. Conclusions: The nano-interaction of Silorane System Adhesive should be attributed to its relatively high pH of 2.7. The obtained tight interface at both enamel and dentin indicates that the two-step self-etch adhesive effectively bridged the hydrophilic tooth substrate with the hydrophobic silorane composite.

AB - Objectives: The low-shrinking composite composed of combined siloxane-oxirane technology (Filtek Silorane, 3M ESPE, Seefeld, Germany) required the development of a specific adhesive (Silorane System Adhesive, 3M ESPE), in particular because of the high hydrophobicity of the silorane composite. The purpose of this study was to characterize the interfacial ultra-structure at enamel and dentin using transmission electron microscopy (TEM). Methods: Non-demineralized/demineralized 70-90 nm sections were prepared following common TEM specimen processing procedures. Results: TEM revealed a typical twofold build-up of the adhesive resin, resulting in a total adhesive layer thickness of 10-20 μm. At bur-cut enamel, a tight interface without distinct dissolution of hydroxyapatite was observed. At bur-cut dentin, a relatively thin hybrid layer of maximum a few hundreds of nanometer was formed without clear surface demineralization. No clear resin tags were formed. At fractured dentin, the interaction appeared very superficial (100-200 nm). Distinct resin tags were formed due to the absence of smear plugs. Silver-nitrate infiltration showed a varying pattern of both spot- and cluster-like appearance of nano-leakage. Traces of Ag were typically detected along some part of the enamel-adhesive interface and/or between the two adhesive resin layers. Substantially more Ag-infiltration was observed along the dentin-adhesive interface of bur-cut dentin, as compared to that of fractured dentin. Conclusions: The nano-interaction of Silorane System Adhesive should be attributed to its relatively high pH of 2.7. The obtained tight interface at both enamel and dentin indicates that the two-step self-etch adhesive effectively bridged the hydrophilic tooth substrate with the hydrophobic silorane composite.

KW - Adhesion

KW - Low-shrinking composite

KW - Nano-interaction

KW - Self-etch adhesive

KW - Silorane

UR - http://www.scopus.com/inward/record.url?scp=77951294799&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77951294799&partnerID=8YFLogxK

U2 - 10.1016/j.dental.2010.01.010

DO - 10.1016/j.dental.2010.01.010

M3 - Article

C2 - 20202675

AN - SCOPUS:77951294799

VL - 26

SP - 524

EP - 532

JO - Dental Materials

JF - Dental Materials

SN - 0109-5641

IS - 6

ER -