Telomerase-specific oncolytic virotherapy for human cancer with the hTERT promoter.

Toshiyoshi Fujiwara, Noriaki Tanaka

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Replication-selective tumor-specific viruses present a novel approach for treatment of neoplastic disease. Telomerase activation is considered to be a critical step in carcinogenesis and its activity correlates closely with human telomerase reverse transcriptase (hTERT) expression. We constructed an attenuated adenovirus 5 vector (Telomelysin, OBP-301), in which the hTERT promoter element drives expression of E1 genes. Telomelysin replicated efficiently and induced marked cell killing in a panel of human cancer cell lines, whereas replication as well as cytotoxicity was highly attenuated in normal human cells lacking telomerase activity. We further modified the E3 region of OBP-301 to contain green fluorescent protein (GFP) gene for monitoring viral replication (TelomeScan, OBP-401). When TelomeScan was intratumorally injected into human tumors orthotopically implanted into the rectum in mice, para-aortic lymph node metastasis could be visualized at laparotomy under a three-chip color cooled charged-coupled device camera. This article reviews recent highlights in this rapidly evolving field: cancer therapeutic and cancer diagnostic approaches using the telomerase-specific oncolytic adenoviruses.

Original languageEnglish
Pages (from-to)11-18
Number of pages8
JournalUirusu. Journal of virology
Volume58
Issue number1
DOIs
Publication statusPublished - Jun 2008

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Oncolytic Virotherapy
Telomerase
Adenoviridae
Neoplasms
Oncogenic Viruses
Viral Genes
Green Fluorescent Proteins
Rectum
Laparotomy
Carcinogenesis
Color
Lymph Nodes
Neoplasm Metastasis
Gene Expression
Cell Line
Equipment and Supplies
human TERT protein
Therapeutics

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Telomerase-specific oncolytic virotherapy for human cancer with the hTERT promoter. / Fujiwara, Toshiyoshi; Tanaka, Noriaki.

In: Uirusu. Journal of virology, Vol. 58, No. 1, 06.2008, p. 11-18.

Research output: Contribution to journalArticle

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