Telomerase reverse transcriptase gene amplification in hepatocellular carcinoma

Yoshitaka Takuma, Kazuhiro Nouso, Yoshiyuki Kobayashi, Shinichiro Nakamura, Hironori Tanaka, Eiji Matsumoto, Tatsuya Fujikawa, Mayumi Suzuki, Tadashi Hanafusa, Yasushi Shiratori

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Background and Aim: Telomerase activation is essential for the immortality of cancer cells. The expression of telomerase reverse transcriptase (hTERT), the catalytic component of the telomerase complex, regulates telomerase activity in human cancers. Amplification of the hTERT gene, located at chromosome 5p, is thought to be a potential genetic event contributing to telomerase activation in sporadic tumors. Methods: The amplification of the hTERT gene was examined in 46 surgically resected hepatocellular carcinomas (HCC) by real-time polymerase chain reaction and the status was compared with the expression of hTERT mRNA and clinicopathological parameters. Results: Amplified hTERT genes were found in 21.7% (10/46) of HCC. The incidence of amplified hTERT genes in poorly differentiated HCC (6/12, 50%) was significantly higher than that in highly to moderately differentiated HCC (4/34, 11.8%; P = 0.012). Tumor size in those cases with hTERT gene amplification was larger compared to those cases with no amplification (P = 0.047). Amplification of the hTERT gene was not observed in non-cancerous tissues. The hTERT mRNA level did not correlate with the number of hTERT genes. Conclusions: Based on these results, it is thought that hTERT gene amplification is a cancer-specific event, and may furthermore contribute to the dedifferentiation and development of HCC. However, hTERT gene overexpression was rarely due to an increased hTERT gene copy number in HCC.

Original languageEnglish
Pages (from-to)1300-1304
Number of pages5
JournalJournal of Gastroenterology and Hepatology (Australia)
Volume19
Issue number11
DOIs
Publication statusPublished - Nov 2004

Keywords

  • Gene amplification
  • Hepatocellular carcinoma
  • Real-time polymerase chain reaction
  • Telomerase reverse transcriptase

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

Fingerprint Dive into the research topics of 'Telomerase reverse transcriptase gene amplification in hepatocellular carcinoma'. Together they form a unique fingerprint.

  • Cite this

    Takuma, Y., Nouso, K., Kobayashi, Y., Nakamura, S., Tanaka, H., Matsumoto, E., Fujikawa, T., Suzuki, M., Hanafusa, T., & Shiratori, Y. (2004). Telomerase reverse transcriptase gene amplification in hepatocellular carcinoma. Journal of Gastroenterology and Hepatology (Australia), 19(11), 1300-1304. https://doi.org/10.1111/j.1440-1746.2004.03447.x