Telomelysin shows potent antitumor activity through apoptotic and non-apoptotic cell death in soft tissue sarcoma cells

Gui Dong Li, Hiroyuki Kawashima, Akira Ogose, Takashi Ariizumi, Tetsuo Hotta, Ryozo Kuwano, Yasuo Urata, Toshiyoshi Fujiwara, Naoto Endo

Research output: Contribution to journalArticle

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Abstract

This study investigated the pathway underlying the antitumor activity of telomelysin, a telomerase-dependent, replication-selective oncolytic adenovirus, in soft tissue sarcoma cells. Treatment with telomelysin alone resulted in simultaneous induction of apoptosis and autophagy, whereas cotreatment with telomelysin and 3-methyladenine significantly reduced cell viability and increased apoptosis and the cellular ATP level compared to treatment with telomelysin alone, indicating that telomelysin-mediated autophagy is a death-protective but not death-promoting process. Cotreatment with Z-Val-Ala-Asp-CH2F significantly increased cellular ATP depletion compared to telomelysin-alone treatment while inhibiting telomelysin-induced apoptosis and having no significant effect on cell viability, indicating that it promotes transition from apoptotic to necrotic cell death.

Original languageEnglish
Pages (from-to)1178-1188
Number of pages11
JournalCancer Science
Volume104
Issue number9
DOIs
Publication statusPublished - Sep 2013

Fingerprint

Sarcoma
Cell Death
Autophagy
Apoptosis
Cell Survival
Adenosine Triphosphate
Telomerase
Adenoviridae
benzyloxycarbonyl-valyl-alanyl-aspartyl-fluoromethane
3-methyladenine

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Li, G. D., Kawashima, H., Ogose, A., Ariizumi, T., Hotta, T., Kuwano, R., ... Endo, N. (2013). Telomelysin shows potent antitumor activity through apoptotic and non-apoptotic cell death in soft tissue sarcoma cells. Cancer Science, 104(9), 1178-1188. https://doi.org/10.1111/cas.12208

Telomelysin shows potent antitumor activity through apoptotic and non-apoptotic cell death in soft tissue sarcoma cells. / Li, Gui Dong; Kawashima, Hiroyuki; Ogose, Akira; Ariizumi, Takashi; Hotta, Tetsuo; Kuwano, Ryozo; Urata, Yasuo; Fujiwara, Toshiyoshi; Endo, Naoto.

In: Cancer Science, Vol. 104, No. 9, 09.2013, p. 1178-1188.

Research output: Contribution to journalArticle

Li, GD, Kawashima, H, Ogose, A, Ariizumi, T, Hotta, T, Kuwano, R, Urata, Y, Fujiwara, T & Endo, N 2013, 'Telomelysin shows potent antitumor activity through apoptotic and non-apoptotic cell death in soft tissue sarcoma cells', Cancer Science, vol. 104, no. 9, pp. 1178-1188. https://doi.org/10.1111/cas.12208
Li, Gui Dong ; Kawashima, Hiroyuki ; Ogose, Akira ; Ariizumi, Takashi ; Hotta, Tetsuo ; Kuwano, Ryozo ; Urata, Yasuo ; Fujiwara, Toshiyoshi ; Endo, Naoto. / Telomelysin shows potent antitumor activity through apoptotic and non-apoptotic cell death in soft tissue sarcoma cells. In: Cancer Science. 2013 ; Vol. 104, No. 9. pp. 1178-1188.
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