Tau pathology in diffuse neurofibrillary tangles with calcification (DNTC): Biochemical and immunohistochemical investigation

Yasuyuki Tanabe, Hideki Ishizu, Koichi Ishiguro, Nobuo Itoh, Seishi Terada, Takashi Haraguchi, Kensuke Kawai, Shigetoshi Kuroda

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

Diffuse neurofibrillary tangles with calcification (DNTC) represents a primary and sporadic presenile dementia that is characterized by temporal or fronto-temporal atrophy with diffuse neurofibrillary, tangles (NFTs), neuropil threads and Fahr-type calcification without senile plaques. We examined the tau pathology in five autopsy cases of DNTC by immunoblotting and immunohistochemistry using phosphorylation-dependent and -independent anti-tau antibodies. The pattern of staining for different epitopes of β-amyloid (Aβ) was also investigated. NFTs were immunopositive with all the anti-tau antibodies used in this study. On the immunoblots, sarkosyl-insoluble tau appeared as three major bands of 60, 64 and 68 kDa, and as a minor band at 72 kDa. The majority of extracellular NFTs were weakly immunopositive only with the antibody recognizing the 40 carboxyl-terminal of Aβ in DNTC. These results suggest that Alzheimer's disease-like tau pathology could exist independently of Aβ deposits in DNTC. (C) 2000 Lippincott Williams and Wilkins.

Original languageEnglish
Pages (from-to)2473-2477
Number of pages5
JournalNeuroReport
Volume11
Issue number11
Publication statusPublished - Aug 3 2000

Keywords

  • Amyloid β protein
  • Calcification
  • Dementia
  • Diffuse neurofibrillary tangles with calcification (DNTC)
  • Extracellular neurofibrillary tangles
  • Tau
  • Tauopathy

ASJC Scopus subject areas

  • Neuroscience(all)

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