Taste responsiveness of fungiform taste cells with action potentials

Ryusuke Yoshida, Noriatsu Shigemura, Keisuke Sanematsu, Keiko Yasumatsu, Satoru Ishizuka, Yuzo Ninomiya

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

It is known that a subset of taste cells generate action potentials in response to taste stimuli. However, responsiveness of these cells to particular tastants remains unknown. In the present study, by using a newly developed extracellular recording technique, we recorded action potentials from the basolateral membrane of single receptor cells in response to taste stimuli applied apically to taste buds isolated from mouse fungiform papillae. By this method, we examined taste-cell responses to stimuli representing the four basic taste qualities (NaCl, Na saccharin, HCl, and quinine-HCl). Of 72 cells responding to taste stimuli, 48 (67%) responded to one, 22 (30%) to two, and 2 (3%) to three of four taste stimuli. The entropy value presenting the breadth of responsiveness was 0.158 ± 0.234 (mean ± SD), which was close to that for the nerve fibers (0.183 ± 0.262). In addition, the proportion of taste cells predominantly sensitive to each of the four taste stimuli, and the grouping of taste cells based on hierarchical cluster analysis, were comparable with those of chorda tympani (CT) fibers. The occurrence of each class of taste cells with different taste responsiveness to the four taste stimuli was not significantly different from that of CT fibers except for classes with broad taste responsiveness. These results suggest that information derived from taste cells generating action potentials may provide the major component of taste information that is transmitted to gustatory nerve fibers.

Original languageEnglish
Pages (from-to)3088-3095
Number of pages8
JournalJournal of Neurophysiology
Volume96
Issue number6
DOIs
Publication statusPublished - Dec 1 2006
Externally publishedYes

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Action Potentials
Nerve Fibers
Taste Buds
Saccharin
Quinine
Entropy
Cluster Analysis

ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology

Cite this

Yoshida, R., Shigemura, N., Sanematsu, K., Yasumatsu, K., Ishizuka, S., & Ninomiya, Y. (2006). Taste responsiveness of fungiform taste cells with action potentials. Journal of Neurophysiology, 96(6), 3088-3095. https://doi.org/10.1152/jn.00409.2006

Taste responsiveness of fungiform taste cells with action potentials. / Yoshida, Ryusuke; Shigemura, Noriatsu; Sanematsu, Keisuke; Yasumatsu, Keiko; Ishizuka, Satoru; Ninomiya, Yuzo.

In: Journal of Neurophysiology, Vol. 96, No. 6, 01.12.2006, p. 3088-3095.

Research output: Contribution to journalArticle

Yoshida, R, Shigemura, N, Sanematsu, K, Yasumatsu, K, Ishizuka, S & Ninomiya, Y 2006, 'Taste responsiveness of fungiform taste cells with action potentials', Journal of Neurophysiology, vol. 96, no. 6, pp. 3088-3095. https://doi.org/10.1152/jn.00409.2006
Yoshida R, Shigemura N, Sanematsu K, Yasumatsu K, Ishizuka S, Ninomiya Y. Taste responsiveness of fungiform taste cells with action potentials. Journal of Neurophysiology. 2006 Dec 1;96(6):3088-3095. https://doi.org/10.1152/jn.00409.2006
Yoshida, Ryusuke ; Shigemura, Noriatsu ; Sanematsu, Keisuke ; Yasumatsu, Keiko ; Ishizuka, Satoru ; Ninomiya, Yuzo. / Taste responsiveness of fungiform taste cells with action potentials. In: Journal of Neurophysiology. 2006 ; Vol. 96, No. 6. pp. 3088-3095.
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