Targeting of post-ischemic cerebral endothelium in rat by liposomes bearing polyethylene glycol-coupled transferrin

Nobuhiko Omori, K. Maruyama, G. Jin, F. Li, S. J. Wang, Y. Hamakawa, K. Sato, I. Nagano, M. Shoji, Koji Abe

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

To achieve an efficient delivery targeting to post-ischemic cerebral vascular endothelium, PEG-liposome conjugated with transferrin (Tf) (Tf-PEG-liposome) was intravenously administered to the rats after 90 min of transient middle cerebral occlusion. The expression of Tf receptor (TfR) in the cerebral endothelium increased with a peak at 1 day after the reperfusion and returned to the control level by 6 days. The Tf-PEG fluorescence was marginally detectable in sham control brain, but remarkably increased with a peak at 2 days, showing about 70% of TfR positive vascular endothelium double-labeled with Tf-PEG. These results indicate that the Tf-PEG-liposome could be utilized as an efficient drug delivery tool to the brain after stroke.

Original languageEnglish
Pages (from-to)275-279
Number of pages5
JournalNeurological Research
Volume25
Issue number3
DOIs
Publication statusPublished - Apr 1 2003

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Transferrin
Liposomes
Endothelium
Vascular Endothelium
Transferrin Receptors
Brain
Reperfusion
Fluorescence
Stroke
Pharmaceutical Preparations

Keywords

  • CM-Dil
  • Drug delivery system
  • Liposome
  • MCAO
  • Transferrin receptor

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Targeting of post-ischemic cerebral endothelium in rat by liposomes bearing polyethylene glycol-coupled transferrin. / Omori, Nobuhiko; Maruyama, K.; Jin, G.; Li, F.; Wang, S. J.; Hamakawa, Y.; Sato, K.; Nagano, I.; Shoji, M.; Abe, Koji.

In: Neurological Research, Vol. 25, No. 3, 01.04.2003, p. 275-279.

Research output: Contribution to journalArticle

Omori, N, Maruyama, K, Jin, G, Li, F, Wang, SJ, Hamakawa, Y, Sato, K, Nagano, I, Shoji, M & Abe, K 2003, 'Targeting of post-ischemic cerebral endothelium in rat by liposomes bearing polyethylene glycol-coupled transferrin', Neurological Research, vol. 25, no. 3, pp. 275-279. https://doi.org/10.1179/016164103101201508
Omori, Nobuhiko ; Maruyama, K. ; Jin, G. ; Li, F. ; Wang, S. J. ; Hamakawa, Y. ; Sato, K. ; Nagano, I. ; Shoji, M. ; Abe, Koji. / Targeting of post-ischemic cerebral endothelium in rat by liposomes bearing polyethylene glycol-coupled transferrin. In: Neurological Research. 2003 ; Vol. 25, No. 3. pp. 275-279.
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AU - Hamakawa, Y.

AU - Sato, K.

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