Targeted genome modifications in cereal crops

Hiroshi Hisano, Fumitaka Abe, Robert E. Hoffie, Jochen Kumlehn

Research output: Contribution to journalReview articlepeer-review

7 Citations (Scopus)


The recent advent of customizable endonucleases has led to remarkable advances in genetic engineering, as these molecular scissors allow for the targeted introduction of mutations or even precisely predefined genetic modifications into virtually any genomic target site of choice. Thanks to its unprecedented precision, effi-ciency, and functional versatility, this technology, commonly referred to as genome editing, has become an effective force not only in basic research devoted to the elucidation of gene function, but also for knowledge-based improvement of crop traits. Among the different platforms currently available for site-directed genome modifications, RNA-guided clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) endonucleases have proven to be the most powerful. This review provides an application-oriented overview of the development of customizable endonucleases, current approaches to cereal crop breeding, and future opportunities in this field.

Original languageEnglish
Pages (from-to)405-416
Number of pages12
JournalBreeding Science
Issue number4
Publication statusPublished - Oct 2021


  • barley
  • Maize
  • Rice
  • Wheat
  • Zinc-finger nucleases

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science


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