Targeted cloning of cytotoxic T cells specific for minor histocompatibility antigens restricted by HLA class I molecules of interest

Yoshiki Akatsuka, Eisei Kondo, Hirohumi Taji, Yasuo Morishima, Makoto Yazaki, Yuichi Obata, Yoshihisa Kodera, Stanley R. Riddell, Toshitada Takahashi

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Abstract

Background. T cells specific for minor histocompatibility antigens (mHAgs) play a major role in both graft-versus-host disease and the graft-versus-tumor effect after HLA-identical bone marrow transplantation (BMT). However, characterization of individual T-cell responses to mHAgs is difficult and has generally involved extensive screening of T-cell clones isolated from bulk T-cell cultures generated from BMT recipients. In this report, we describe a new approach that permits both direct visualization of CD8+ T-cell responses to mHAgs and cloning of T cells reacting with mHAgs presented by individual HLA alleles of interest. Methods and Results. Panels of Epstein-Barr virus-transformed B-cell lines (B-LCL) expressing retrovirally transduced HLA cDNA were used as stimulator cells in an enzyme-linked immunospot (ELISPOT) assay to identify CD8+ T cells reacting with mHAgs in cultures generated from postBMT recipient peripheral blood. T cells specific for mHAgs presented by selected HLA alleles could then be captured and cloned using an interferon-y secretion assay and magnetic bead selection. A majority of T-cell clones thus isolated exhibited cytolytic activity against the same HLA-transfected B-cell lines used for the ELISPOT assay. Conclusion. The ELISPOT assay was useful for identification of the HLA alleles presenting mHAgs recognized by individual T-cell lines. This approach for isolating mHAgs-specific CD8+ T-cell clones should assist in characterizing responses restricted by HLA alleles of interest, which are common in a certain ethnic group.

Original languageEnglish
Pages (from-to)1773-1780
Number of pages8
JournalTransplantation
Volume74
Issue number12
DOIs
Publication statusPublished - Dec 27 2002

ASJC Scopus subject areas

  • Transplantation

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    Akatsuka, Y., Kondo, E., Taji, H., Morishima, Y., Yazaki, M., Obata, Y., Kodera, Y., Riddell, S. R., & Takahashi, T. (2002). Targeted cloning of cytotoxic T cells specific for minor histocompatibility antigens restricted by HLA class I molecules of interest. Transplantation, 74(12), 1773-1780. https://doi.org/10.1097/00007890-200212270-00023