Target protein discrimination based on array sensor using calcein-encapsulating liposomes with cholesterol by principal component analysis

R. Imamura, Z. Zhang, T. Yoshikawa, Toshinori Shimanouchi, N. Murata, K. Yamashita, M. Fukuzawa, M. Noda

Research output: Contribution to journalArticle

Abstract

A bio array sensing system is newly composed utilizing different phospholipid liposomes encapsulating fluorescent molecules. We have confirmed a high output intensity of fluorescence emission due to the characteristics dependent on the concentration of fluorescent molecules when the fluorescent molecules leak from inside of the liposome through perturbed lipid membrane. After measuring a whole array image of fluorescence emission output from every element of liposome sensor by a new CMOS imager system, the outputs of fluorescence emission from all the elements were analyzed by a statistical method of principal component analysis (PCA). It was found from obtained PCA plots that different species of proteins with several concentrations were clearly discriminated with high cumulative contribution ratio.

Original languageEnglish
Pages (from-to)699-702
Number of pages4
JournalProcedia Engineering
Volume120
DOIs
Publication statusPublished - 2015

Keywords

  • Array
  • Biosensor
  • Cholesterol
  • Fluorescence
  • Liposome
  • Principal component analysis
  • Protein

ASJC Scopus subject areas

  • Engineering(all)

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