Tandem repetition of baculovirus ie1 promoter results in upregulation of transcription

K. Kojima, Tohru Hayakawa, S. Asano, H. Bando

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In order to improve a virus-independent gene expression system in insect cells, the effect of the repetition of the promoter sequence of a baculovirus immediate early gene, BmNPV ie1, on the gene expression activity was examined. Tandem repetition of ie1 promoter resulted in about a 4-fold increase of the reporter gene expression activity. Primer extension analysis revealed that the repetition increased not only the amount of transcripts (4 to 5-times) but also the number of sites of transcription initiation (at least 3 extra initiation sites). A mutational experiment demonstrated the involvement of independent cis-acting elements in these two different promoter-enhancing effects.

Original languageEnglish
Pages (from-to)1407-1414
Number of pages8
JournalArchives of Virology
Volume146
Issue number7
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Baculoviridae
Up-Regulation
Gene Expression
Immediate-Early Genes
Transcription Initiation Site
Reporter Genes
Insects
Viruses

ASJC Scopus subject areas

  • Genetics
  • Applied Microbiology and Biotechnology

Cite this

Tandem repetition of baculovirus ie1 promoter results in upregulation of transcription. / Kojima, K.; Hayakawa, Tohru; Asano, S.; Bando, H.

In: Archives of Virology, Vol. 146, No. 7, 2001, p. 1407-1414.

Research output: Contribution to journalArticle

Kojima, K. ; Hayakawa, Tohru ; Asano, S. ; Bando, H. / Tandem repetition of baculovirus ie1 promoter results in upregulation of transcription. In: Archives of Virology. 2001 ; Vol. 146, No. 7. pp. 1407-1414.
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