Abstract
In order to improve a virus-independent gene expression system in insect cells, the effect of the repetition of the promoter sequence of a baculovirus immediate early gene, BmNPV ie1, on the gene expression activity was examined. Tandem repetition of ie1 promoter resulted in about a 4-fold increase of the reporter gene expression activity. Primer extension analysis revealed that the repetition increased not only the amount of transcripts (4 to 5-times) but also the number of sites of transcription initiation (at least 3 extra initiation sites). A mutational experiment demonstrated the involvement of independent cis-acting elements in these two different promoter-enhancing effects.
| Original language | English |
|---|---|
| Pages (from-to) | 1407-1414 |
| Number of pages | 8 |
| Journal | Archives of Virology |
| Volume | 146 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 2001 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Genetics
- Applied Microbiology and Biotechnology
Cite this
Tandem repetition of baculovirus ie1 promoter results in upregulation of transcription. / Kojima, K.; Hayakawa, Tohru; Asano, S.; Bando, H.
In: Archives of Virology, Vol. 146, No. 7, 2001, p. 1407-1414.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Tandem repetition of baculovirus ie1 promoter results in upregulation of transcription
AU - Kojima, K.
AU - Hayakawa, Tohru
AU - Asano, S.
AU - Bando, H.
PY - 2001
Y1 - 2001
N2 - In order to improve a virus-independent gene expression system in insect cells, the effect of the repetition of the promoter sequence of a baculovirus immediate early gene, BmNPV ie1, on the gene expression activity was examined. Tandem repetition of ie1 promoter resulted in about a 4-fold increase of the reporter gene expression activity. Primer extension analysis revealed that the repetition increased not only the amount of transcripts (4 to 5-times) but also the number of sites of transcription initiation (at least 3 extra initiation sites). A mutational experiment demonstrated the involvement of independent cis-acting elements in these two different promoter-enhancing effects.
AB - In order to improve a virus-independent gene expression system in insect cells, the effect of the repetition of the promoter sequence of a baculovirus immediate early gene, BmNPV ie1, on the gene expression activity was examined. Tandem repetition of ie1 promoter resulted in about a 4-fold increase of the reporter gene expression activity. Primer extension analysis revealed that the repetition increased not only the amount of transcripts (4 to 5-times) but also the number of sites of transcription initiation (at least 3 extra initiation sites). A mutational experiment demonstrated the involvement of independent cis-acting elements in these two different promoter-enhancing effects.
UR - http://www.scopus.com/inward/record.url?scp=0034905233&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034905233&partnerID=8YFLogxK
U2 - 10.1007/s007050170101
DO - 10.1007/s007050170101
M3 - Article
C2 - 11556716
AN - SCOPUS:0034905233
VL - 146
SP - 1407
EP - 1414
JO - Archives of Virology
JF - Archives of Virology
SN - 0304-8608
IS - 7
ER -