T cell responses to major membrane protein II (MMP II) of Mycobacterium leprae are restricted by HLA-DR molecules in patients with leprosy

Hideki Ohyama, Sho Matsushita, Fusanori Nishimura, Nahoko Kato, Kentaro Hatano, Shogo Takashiba, Yoji Murayama

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Major membrane protein II (MMP II) of Mycobacterium leprae (M. leprae) is a 22kDa protein inducing humoral immune response in leprosy patients. MMP II-specific bulk T cell lines were established from leprosy patients to determine major T cell epitopes in MMP II and to evaluate lymphokine production induced by MMP II. These bulk T cell lines reacted to one or more peptides in the locus of amino acid residues from 23 to 109 of MMP II. The proliferative responses of all T cell lines were mainly inhibited by the addition of anti-DRB1 mAb. Many bulk T cell lines induced IFN-γ, IL-5, but not IL-4. However, it was not possible to distinguish the LL or TT types of leprosy based on the pattern of T cell epitopes and the lymphokine productivity in the responses against MMP II. Thus, it appears that T cell response to MMP II is restricted by the HLA-DRB1 molecule, but not by DQ and DP molecules, which results in the induction of IFN-γ production.

Original languageEnglish
Pages (from-to)475-482
Number of pages8
JournalVaccine
Volume20
Issue number3-4
DOIs
Publication statusPublished - Nov 12 2001

Keywords

  • Class II HLA
  • Leprosy patients
  • T cell responses

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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