T cell responses to 53-kDa outer membrane protein of Porphyromonas gingivalis in humans with early-onset periodontitis

Hideki Ohyama, Sho Matsushita, Nahoko Kato, Fusanori Nishimura, Kosuke Oyaizu, Susumu Kokeguchi, Hidemi Kurihara, Shogo Takashiba, Yasuharu Nishimura, Yoji Murayama

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Patients with early-onset periodontitis (EOP) are susceptible to infection with periodontopathic bacteria, such as Porphyromonas gingivalis. Ag53, 53-kDa outer membrane protein of P. gingivalis, evokes strong humoral immune responses in EOP patients. In a first step to clarify how host immune cells recognize Ag53, we established Ag53-specific short-term T cell lines from 22 subjects including 6 EOP patients and 16 healthy donors, using overlapping peptides based on Ag53 amino acid sequences. All T cell lines from active EOP patients recognized a common region (p141-181, especially p141-161) on Ag53, while those from healthy donors showed heterogeneous specificity. p141-181 was not recognized by T cell lines established from EOP patients following therapy. A monoclonal antibody to HLA-DRB1 inhibited Ag53- induced proliferation of most of the T cell lines. Our observations suggest that, although antigen-presenting molecules are common in EOP patients and in healthy individuals, p141-161 includes a major T cell epitope(s) on Ag53 for active EOP patients but not for healthy individuals or inactive EOP patients.

Original languageEnglish
Pages (from-to)635-643
Number of pages9
JournalHuman Immunology
Volume59
Issue number10
DOIs
Publication statusPublished - Oct 1998

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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