Abstract
Human thymoma is derived from thymic epithelial cells and often associated with a large number of conical thymocytes. Since thymic epithelial cells play key roles in T-cell development in the normal thymus, we hypothesized that the neoplastic epithelial cells of thymoma may support T-cell differentiation. We attempted to reconstitute the T-cell development in vitro by using neoplastic epithelial cells isolated from thymoma. CD34, a stem cell marker, was expressed on a proportion of CD4-CD8- cells in thymoma. These CD34+CD4-CD8- cells also expressed both IL-7R α-chain and common γ-chain. Purified CD4-CD8- cells from thymomas were cultured with the neoplastic epithelial cells, and their differentiation into CD4+CD8+ cells via CD4 single positive intermediates was observed within 9 days' co-culture in the presence of recombinant IL-7. The CD34+CD4-CD8- cells purified from a normal thymus also differentiated to CD4+CD8+ cells in an allogeneic co-culture with the neoplastic epithelial cells of thymoma. In addition, a pleural dissemination from thymoma contained a large amount of cortical thymocytes. These results suggest that the neoplastic epithelial cells retain the function of thymic epithelium and can support T-cell development in thymomas.
| Original language | English |
|---|---|
| Pages (from-to) | 541-547 |
| Number of pages | 7 |
| Journal | Pathology Research and Practice |
| Volume | 195 |
| Issue number | 8 |
| Publication status | Published - 1999 |
| Externally published | Yes |
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Keywords
- Human
- T-cell development
- Thymic epithelial cell
- Thymoma
ASJC Scopus subject areas
- Pathology and Forensic Medicine
Cite this
T-cell development in human thymoma. / Inoue, Masayoshi; Fujii, Yoshitaka; Okumura, Meinoshin; Miyoshi, Shinichiro; Shiono, Hiroyuki; Fukuhara, Kenjiro; Kadota, Yoshihisa; Matsuda, Hikaru.
In: Pathology Research and Practice, Vol. 195, No. 8, 1999, p. 541-547.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - T-cell development in human thymoma
AU - Inoue, Masayoshi
AU - Fujii, Yoshitaka
AU - Okumura, Meinoshin
AU - Miyoshi, Shinichiro
AU - Shiono, Hiroyuki
AU - Fukuhara, Kenjiro
AU - Kadota, Yoshihisa
AU - Matsuda, Hikaru
PY - 1999
Y1 - 1999
N2 - Human thymoma is derived from thymic epithelial cells and often associated with a large number of conical thymocytes. Since thymic epithelial cells play key roles in T-cell development in the normal thymus, we hypothesized that the neoplastic epithelial cells of thymoma may support T-cell differentiation. We attempted to reconstitute the T-cell development in vitro by using neoplastic epithelial cells isolated from thymoma. CD34, a stem cell marker, was expressed on a proportion of CD4-CD8- cells in thymoma. These CD34+CD4-CD8- cells also expressed both IL-7R α-chain and common γ-chain. Purified CD4-CD8- cells from thymomas were cultured with the neoplastic epithelial cells, and their differentiation into CD4+CD8+ cells via CD4 single positive intermediates was observed within 9 days' co-culture in the presence of recombinant IL-7. The CD34+CD4-CD8- cells purified from a normal thymus also differentiated to CD4+CD8+ cells in an allogeneic co-culture with the neoplastic epithelial cells of thymoma. In addition, a pleural dissemination from thymoma contained a large amount of cortical thymocytes. These results suggest that the neoplastic epithelial cells retain the function of thymic epithelium and can support T-cell development in thymomas.
AB - Human thymoma is derived from thymic epithelial cells and often associated with a large number of conical thymocytes. Since thymic epithelial cells play key roles in T-cell development in the normal thymus, we hypothesized that the neoplastic epithelial cells of thymoma may support T-cell differentiation. We attempted to reconstitute the T-cell development in vitro by using neoplastic epithelial cells isolated from thymoma. CD34, a stem cell marker, was expressed on a proportion of CD4-CD8- cells in thymoma. These CD34+CD4-CD8- cells also expressed both IL-7R α-chain and common γ-chain. Purified CD4-CD8- cells from thymomas were cultured with the neoplastic epithelial cells, and their differentiation into CD4+CD8+ cells via CD4 single positive intermediates was observed within 9 days' co-culture in the presence of recombinant IL-7. The CD34+CD4-CD8- cells purified from a normal thymus also differentiated to CD4+CD8+ cells in an allogeneic co-culture with the neoplastic epithelial cells of thymoma. In addition, a pleural dissemination from thymoma contained a large amount of cortical thymocytes. These results suggest that the neoplastic epithelial cells retain the function of thymic epithelium and can support T-cell development in thymomas.
KW - Human
KW - T-cell development
KW - Thymic epithelial cell
KW - Thymoma
UR - http://www.scopus.com/inward/record.url?scp=0032856706&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032856706&partnerID=8YFLogxK
M3 - Article
C2 - 10483584
AN - SCOPUS:0032856706
VL - 195
SP - 541
EP - 547
JO - Pathology Research and Practice
JF - Pathology Research and Practice
SN - 0344-0338
IS - 8
ER -