T-cell development in human thymoma

Masayoshi Inoue, Yoshitaka Fujii, Meinoshin Okumura, Shinichiro Miyoshi, Hiroyuki Shiono, Kenjiro Fukuhara, Yoshihisa Kadota, Hikaru Matsuda

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Human thymoma is derived from thymic epithelial cells and often associated with a large number of conical thymocytes. Since thymic epithelial cells play key roles in T-cell development in the normal thymus, we hypothesized that the neoplastic epithelial cells of thymoma may support T-cell differentiation. We attempted to reconstitute the T-cell development in vitro by using neoplastic epithelial cells isolated from thymoma. CD34, a stem cell marker, was expressed on a proportion of CD4-CD8- cells in thymoma. These CD34+CD4-CD8- cells also expressed both IL-7R α-chain and common γ-chain. Purified CD4-CD8- cells from thymomas were cultured with the neoplastic epithelial cells, and their differentiation into CD4+CD8+ cells via CD4 single positive intermediates was observed within 9 days' co-culture in the presence of recombinant IL-7. The CD34+CD4-CD8- cells purified from a normal thymus also differentiated to CD4+CD8+ cells in an allogeneic co-culture with the neoplastic epithelial cells of thymoma. In addition, a pleural dissemination from thymoma contained a large amount of cortical thymocytes. These results suggest that the neoplastic epithelial cells retain the function of thymic epithelium and can support T-cell development in thymomas.

Original languageEnglish
Pages (from-to)541-547
Number of pages7
JournalPathology Research and Practice
Volume195
Issue number8
Publication statusPublished - 1999
Externally publishedYes

Fingerprint

Thymoma
Human Development
T-Lymphocytes
Epithelial Cells
Thymocytes
Coculture Techniques
Thymus Gland
Cell Differentiation
Interleukin-7
Stem Cells
Epithelium

Keywords

  • Human
  • T-cell development
  • Thymic epithelial cell
  • Thymoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Inoue, M., Fujii, Y., Okumura, M., Miyoshi, S., Shiono, H., Fukuhara, K., ... Matsuda, H. (1999). T-cell development in human thymoma. Pathology Research and Practice, 195(8), 541-547.

T-cell development in human thymoma. / Inoue, Masayoshi; Fujii, Yoshitaka; Okumura, Meinoshin; Miyoshi, Shinichiro; Shiono, Hiroyuki; Fukuhara, Kenjiro; Kadota, Yoshihisa; Matsuda, Hikaru.

In: Pathology Research and Practice, Vol. 195, No. 8, 1999, p. 541-547.

Research output: Contribution to journalArticle

Inoue, M, Fujii, Y, Okumura, M, Miyoshi, S, Shiono, H, Fukuhara, K, Kadota, Y & Matsuda, H 1999, 'T-cell development in human thymoma', Pathology Research and Practice, vol. 195, no. 8, pp. 541-547.
Inoue M, Fujii Y, Okumura M, Miyoshi S, Shiono H, Fukuhara K et al. T-cell development in human thymoma. Pathology Research and Practice. 1999;195(8):541-547.
Inoue, Masayoshi ; Fujii, Yoshitaka ; Okumura, Meinoshin ; Miyoshi, Shinichiro ; Shiono, Hiroyuki ; Fukuhara, Kenjiro ; Kadota, Yoshihisa ; Matsuda, Hikaru. / T-cell development in human thymoma. In: Pathology Research and Practice. 1999 ; Vol. 195, No. 8. pp. 541-547.
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