T-armless tRNAs and elongated elongation factor Tu

Takashi Ohtsuki, Yoh Ichi Watanabe

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Most tRNAs share a common secondary structure containing a T arm, a D arm, an anticodon arm and an acceptor stem. However, there are some exceptions. Most nematode mitochondrial tRNAs and some animal mitochondrial tRNAs lack the T arm, which is necessary for binding to canonical elongation factor Tu (EF-Tu). The mitochondria of the nematode Caenorhabditis elegans have a unique EF-Tu, named EF-Tu1, whose structure has supplied clues as to how truncated tRNAs can work in translation. EF-Tu1 has a C-terminal extension of about 60 aa that is absent in canonical EF-Tu. Recent data from our laboratory strongly suggests that EF-Tu1 recognizes the D-arm instead of the T arm by a mechanism involving this C-terminal region. Further biochemical analysis of mitochondrial tRNAs and EF-Tu from the distantly related nematode Trichinella spp. and sequence information on nuclear and mitochondrial DNA in arthropods suggest that T-armless tRNAs may have arisen as a result of duplication of the EF-Tu gene. These studies provide valuable insights into the co-evolution of RNA and RNA-binding proteins.

Original languageEnglish
Pages (from-to)68-75
Number of pages8
JournalIUBMB Life
Volume59
Issue number2
DOIs
Publication statusPublished - 2007

Fingerprint

Peptide Elongation Factor Tu
Transfer RNA
Trichinella
Anticodon
Mitochondria
RNA-Binding Proteins
Arthropods
Caenorhabditis elegans
Mitochondrial DNA
Animals
Genes
RNA

Keywords

  • Elongation factor (EF-)Tu
  • Nematode mitochondria
  • T-armless tRNA genes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

T-armless tRNAs and elongated elongation factor Tu. / Ohtsuki, Takashi; Watanabe, Yoh Ichi.

In: IUBMB Life, Vol. 59, No. 2, 2007, p. 68-75.

Research output: Contribution to journalArticle

Ohtsuki, Takashi ; Watanabe, Yoh Ichi. / T-armless tRNAs and elongated elongation factor Tu. In: IUBMB Life. 2007 ; Vol. 59, No. 2. pp. 68-75.
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