SYT, a partner of SYT-SSX oncoprotein in synovial sarcomas, interacts with mSin3A, a component of histone deacetylase complex

Tatsuo Ito, Mamoru Oouchida, Sachio Itou, Yoshimi Jitsumori, Yuki Morimoto, Toshihumi Ozaki, Akira Kawai, Hajime Inoue, Kenji Shimizu

Research output: Contribution to journalArticle

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Abstract

Synovial sarcomas are soft-tissue tumors predominantly affecting children and young adults. They are molecular-genetically characterized by the SYT-SSX fusion gene generated from chromosomal translocation t(X; 18) (p11-2; q11.2). When we screened new gene products that interact with SYT or SSX proteins by yeast two-hybrid assay, we found that mSin3A, a component of the histone deacetylase complex, interacts with SYT but not with SSX. These results were confirmed by mammalian two-hybrid and pull-down assays. Analyses with sequential truncated proteins revealed a main mSin3A-interaction region on the SYT amino-terminal 93 amino acids, and another one on the region between 187th amino acid and break point. In luciferase assay, mSin3A repressed the transcriptional activity of reporter promoter mediated by SYT and hBRM/BRG1. Our results suggest that the histone deacetylase complex containing mSin3A may regulate the transcriptional activation mediated by SYT.

Original languageEnglish
Pages (from-to)1484-1490
Number of pages7
JournalLaboratory Investigation
Volume84
Issue number11
DOIs
Publication statusPublished - Nov 2004

Fingerprint

Synovial Sarcoma
Histone Deacetylases
Oncogene Proteins
Amino Acids
Genetic Translocation
Two-Hybrid System Techniques
Gene Fusion
Luciferases
Transcriptional Activation
Young Adult
Proteins
Genes
Neoplasms

Keywords

  • Chromosomal translocation
  • Histone deacetylation
  • mSin3A
  • Synovial sarcoma
  • SYT
  • Two-hybrid assay

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

SYT, a partner of SYT-SSX oncoprotein in synovial sarcomas, interacts with mSin3A, a component of histone deacetylase complex. / Ito, Tatsuo; Oouchida, Mamoru; Itou, Sachio; Jitsumori, Yoshimi; Morimoto, Yuki; Ozaki, Toshihumi; Kawai, Akira; Inoue, Hajime; Shimizu, Kenji.

In: Laboratory Investigation, Vol. 84, No. 11, 11.2004, p. 1484-1490.

Research output: Contribution to journalArticle

Ito, Tatsuo ; Oouchida, Mamoru ; Itou, Sachio ; Jitsumori, Yoshimi ; Morimoto, Yuki ; Ozaki, Toshihumi ; Kawai, Akira ; Inoue, Hajime ; Shimizu, Kenji. / SYT, a partner of SYT-SSX oncoprotein in synovial sarcomas, interacts with mSin3A, a component of histone deacetylase complex. In: Laboratory Investigation. 2004 ; Vol. 84, No. 11. pp. 1484-1490.
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