SYT, a partner of SYT-SSX oncoprotein in synovial sarcomas, interacts with mSin3A, a component of histone deacetylase complex

Tatsuo Ito, Mamoru Ouchida, Sachio Ito, Yoshimi Jitsumori, Yuki Morimoto, Toshifumi Ozaki, Akira Kawai, Hajime Inoue, Kenji Shimizu

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Synovial sarcomas are soft-tissue tumors predominantly affecting children and young adults. They are molecular-genetically characterized by the SYT-SSX fusion gene generated from chromosomal translocation t(X; 18) (p11-2; q11.2). When we screened new gene products that interact with SYT or SSX proteins by yeast two-hybrid assay, we found that mSin3A, a component of the histone deacetylase complex, interacts with SYT but not with SSX. These results were confirmed by mammalian two-hybrid and pull-down assays. Analyses with sequential truncated proteins revealed a main mSin3A-interaction region on the SYT amino-terminal 93 amino acids, and another one on the region between 187th amino acid and break point. In luciferase assay, mSin3A repressed the transcriptional activity of reporter promoter mediated by SYT and hBRM/BRG1. Our results suggest that the histone deacetylase complex containing mSin3A may regulate the transcriptional activation mediated by SYT.

Original languageEnglish
Pages (from-to)1484-1490
Number of pages7
JournalLaboratory Investigation
Volume84
Issue number11
DOIs
Publication statusPublished - Nov 2004

Keywords

  • Chromosomal translocation
  • Histone deacetylation
  • SYT
  • Synovial sarcoma
  • Two-hybrid assay
  • mSin3A

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

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