Systematic analysis of the relation of electron transport and ATP synthesis to the photodamage and repair of photosystem II in synechocystis

Suleyman Allakhverdiev, Yoshitaka Nishiyama, Shunichi Takahashi, Sachio Miyairi, Iwane Suzuki, Norio Murata

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

The photosynthetic machinery and, in particular, the photosystem II (PSII) complex are susceptible to strong light, and the effects of strong light are referred to as photodamage or photoinhibition. In living organisms, photodamaged PSII is rapidly repaired and, as a result, the extent of photoinhibition represents a balance between rates of photodamage and the repair of PSII. In this study, we examined the roles of electron transport and ATP synthesis in these two processes by monitoring them separately and systematically in the cyanobacterium Synechocystis sp. PCC 6803. We found that the rate of photodamage, which was proportional to light intensity, was unaffected by inhibition of the electron transport in PSII, by acceleration of electron transport in PSI, and by inhibition of ATP synthesis. By contrast, the rate of repair was reduced upon inhibition of the synthesis of ATP either via PSI or PSII. Northern blotting and radiolabeling analysis with [35S]Met revealed that synthesis of the D1 protein was enhanced by the synthesis of ATP. Our observations suggest that ATP synthesis might regulate the repair of PSII, in particular, at the level of translation of the psbA genes for the precursor to the D1 protein, whereas neither electron transport nor the synthesis of ATP affects the extent of photodamage.

Original languageEnglish
Pages (from-to)263-273
Number of pages11
JournalPlant Physiology
Volume137
Issue number1
DOIs
Publication statusPublished - Dec 1 2005
Externally publishedYes

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Synechocystis
Photosystem II Protein Complex
Electron Transport
photosystem II
electron transfer
Adenosine Triphosphate
synthesis
D1 protein
Light
photoinhibition
Synechocystis sp. PCC 6803
Cyanobacteria
radiolabeling
Northern Blotting
Northern blotting
Proteins
translation (genetics)
light intensity
monitoring
organisms

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

Systematic analysis of the relation of electron transport and ATP synthesis to the photodamage and repair of photosystem II in synechocystis. / Allakhverdiev, Suleyman; Nishiyama, Yoshitaka; Takahashi, Shunichi; Miyairi, Sachio; Suzuki, Iwane; Murata, Norio.

In: Plant Physiology, Vol. 137, No. 1, 01.12.2005, p. 263-273.

Research output: Contribution to journalArticle

Allakhverdiev, Suleyman ; Nishiyama, Yoshitaka ; Takahashi, Shunichi ; Miyairi, Sachio ; Suzuki, Iwane ; Murata, Norio. / Systematic analysis of the relation of electron transport and ATP synthesis to the photodamage and repair of photosystem II in synechocystis. In: Plant Physiology. 2005 ; Vol. 137, No. 1. pp. 263-273.
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