Systematic analysis of rice (Oryza sativa) metabolic responses to herbivory

Kabir Md Alamgir, Yuko Hojo, John T. Christeller, Kaori Fukumoto, Ryutaro Isshiki, Tomonori Shinya, Ian T. Baldwin, Ivan Galis

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Plants defend against attack from herbivores by direct and indirect defence mechanisms mediated by the accumulation of phytoalexins and release of volatile signals, respectively. While the defensive arsenals of some plants, such as tobacco and Arabidopsis are well known, most of rice's (Oryza sativa) defence metabolites and their effectiveness against herbivores remain uncharacterized. Here, we used a non-biassed metabolomics approach to identify many novel herbivory-regulated metabolic signatures in rice. Most were up-regulated by herbivore attack while only a few were suppressed. Two of the most prominent up-regulated signatures were characterized as phenolamides (PAs), p-coumaroylputrescine and feruloylputrescine. PAs accumulated in response to attack by both chewing insects, i.e. feeding of the lawn armyworm (Spodoptera mauritia) and the rice skipper (Parnara guttata) larvae, and the attack of the sucking insect, the brown planthopper (Nilaparvata lugens, BPH). In bioassays, BPH insects feeding on 15% sugar solution containing p-coumaroylputrescine or feruloylputrescine, at concentrations similar to those elicited by heavy BPH attack in rice, had a higher mortality compared to those feeding on sugar diet alone. Our results highlight PAs as a rapidly expanding new group of plant defence metabolites that are elicited by herbivore attack, and deter herbivores in rice and other plants.

Original languageEnglish
Pages (from-to)453-466
Number of pages14
JournalPlant, Cell and Environment
Volume39
Issue number2
DOIs
Publication statusPublished - Feb 1 2016

Fingerprint

Herbivory
Oryza sativa
herbivores
rice
Spodoptera mauritia
Insects
Nilaparvata lugens
insects
metabolites
Hesperiidae
sugars
Spodoptera
Metabolomics
Mastication
phytoalexins
metabolomics
Defense Mechanisms
mastication
defense mechanisms
Arabidopsis

Keywords

  • Defence
  • Diterpene phytoalexins (momilactones)
  • Feruloylputrescine (FP)
  • Herbivore damage
  • Metabolomics
  • p-coumaroylputrescine (CoP)
  • Phenolamides (PAs)
  • Rice (Oryza sativa)

ASJC Scopus subject areas

  • Plant Science
  • Physiology

Cite this

Systematic analysis of rice (Oryza sativa) metabolic responses to herbivory. / Alamgir, Kabir Md; Hojo, Yuko; Christeller, John T.; Fukumoto, Kaori; Isshiki, Ryutaro; Shinya, Tomonori; Baldwin, Ian T.; Galis, Ivan.

In: Plant, Cell and Environment, Vol. 39, No. 2, 01.02.2016, p. 453-466.

Research output: Contribution to journalArticle

Alamgir, KM, Hojo, Y, Christeller, JT, Fukumoto, K, Isshiki, R, Shinya, T, Baldwin, IT & Galis, I 2016, 'Systematic analysis of rice (Oryza sativa) metabolic responses to herbivory', Plant, Cell and Environment, vol. 39, no. 2, pp. 453-466. https://doi.org/10.1111/pce.12640
Alamgir, Kabir Md ; Hojo, Yuko ; Christeller, John T. ; Fukumoto, Kaori ; Isshiki, Ryutaro ; Shinya, Tomonori ; Baldwin, Ian T. ; Galis, Ivan. / Systematic analysis of rice (Oryza sativa) metabolic responses to herbivory. In: Plant, Cell and Environment. 2016 ; Vol. 39, No. 2. pp. 453-466.
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