Abstract
The regulation of cell signaling pathways and the reconstruction of genetic circuits are important aspects of bioengineering research. Both of these goals require molecular devices to transmit information from an input biomacromolecule to the desired outputs. Here, we show that an RNA-protein (RNP)-containing L7Ae-kink-turn interaction can be used to construct translational regulators under control of an input protein that regulates the expression of desired output proteins. We built a system in which L7Ae, an archaeal ribosomal protein, regulates the translation of a designed mRNA in vitro and in human cells. The translational regulator composed of the RNP might provide new therapeutic strategies based on the detection, repair or rewiring of intrinsic cellular defects, and it may also serve as an invaluable tool for the dissection of the behavior of complex, higher-order circuits in the cell.
| Original language | English |
|---|---|
| Pages (from-to) | 71-78 |
| Number of pages | 8 |
| Journal | Nature Chemical Biology |
| Volume | 6 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 2010 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cell Biology
- Molecular Biology
Cite this
Synthetic translational regulation by an L7Ae-kink-turn RNP switch. / Saito, Hirohide; Kobayashi, Tetsuhiro; Hara, Tomoaki; Fujita, Yoshihiko; Hayashi, Karin; Furushima, Rie; Inoue, Tan.
In: Nature Chemical Biology, Vol. 6, No. 1, 01.2010, p. 71-78.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Synthetic translational regulation by an L7Ae-kink-turn RNP switch
AU - Saito, Hirohide
AU - Kobayashi, Tetsuhiro
AU - Hara, Tomoaki
AU - Fujita, Yoshihiko
AU - Hayashi, Karin
AU - Furushima, Rie
AU - Inoue, Tan
PY - 2010/1
Y1 - 2010/1
N2 - The regulation of cell signaling pathways and the reconstruction of genetic circuits are important aspects of bioengineering research. Both of these goals require molecular devices to transmit information from an input biomacromolecule to the desired outputs. Here, we show that an RNA-protein (RNP)-containing L7Ae-kink-turn interaction can be used to construct translational regulators under control of an input protein that regulates the expression of desired output proteins. We built a system in which L7Ae, an archaeal ribosomal protein, regulates the translation of a designed mRNA in vitro and in human cells. The translational regulator composed of the RNP might provide new therapeutic strategies based on the detection, repair or rewiring of intrinsic cellular defects, and it may also serve as an invaluable tool for the dissection of the behavior of complex, higher-order circuits in the cell.
AB - The regulation of cell signaling pathways and the reconstruction of genetic circuits are important aspects of bioengineering research. Both of these goals require molecular devices to transmit information from an input biomacromolecule to the desired outputs. Here, we show that an RNA-protein (RNP)-containing L7Ae-kink-turn interaction can be used to construct translational regulators under control of an input protein that regulates the expression of desired output proteins. We built a system in which L7Ae, an archaeal ribosomal protein, regulates the translation of a designed mRNA in vitro and in human cells. The translational regulator composed of the RNP might provide new therapeutic strategies based on the detection, repair or rewiring of intrinsic cellular defects, and it may also serve as an invaluable tool for the dissection of the behavior of complex, higher-order circuits in the cell.
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UR - http://www.scopus.com/inward/citedby.url?scp=77950422751&partnerID=8YFLogxK
U2 - 10.1038/nchembio.273
DO - 10.1038/nchembio.273
M3 - Article
VL - 6
SP - 71
EP - 78
JO - Nature Chemical Biology
JF - Nature Chemical Biology
SN - 1552-4450
IS - 1
ER -