Synthetic translational regulation by an L7Ae-kink-turn RNP switch

Hirohide Saito, Tetsuhiro Kobayashi, Tomoaki Hara, Yoshihiko Fujita, Karin Hayashi, Rie Furushima, Tan Inoue

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

The regulation of cell signaling pathways and the reconstruction of genetic circuits are important aspects of bioengineering research. Both of these goals require molecular devices to transmit information from an input biomacromolecule to the desired outputs. Here, we show that an RNA-protein (RNP)-containing L7Ae-kink-turn interaction can be used to construct translational regulators under control of an input protein that regulates the expression of desired output proteins. We built a system in which L7Ae, an archaeal ribosomal protein, regulates the translation of a designed mRNA in vitro and in human cells. The translational regulator composed of the RNP might provide new therapeutic strategies based on the detection, repair or rewiring of intrinsic cellular defects, and it may also serve as an invaluable tool for the dissection of the behavior of complex, higher-order circuits in the cell.

Original languageEnglish
Pages (from-to)71-78
Number of pages8
JournalNature Chemical Biology
Volume6
Issue number1
DOIs
Publication statusPublished - Jan 2010
Externally publishedYes

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RNA
Proteins
Archaeal Proteins
Bioengineering
Ribosomal Proteins
Protein Biosynthesis
Dissection
Equipment and Supplies
Messenger RNA
Research
Therapeutics
In Vitro Techniques

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Saito, H., Kobayashi, T., Hara, T., Fujita, Y., Hayashi, K., Furushima, R., & Inoue, T. (2010). Synthetic translational regulation by an L7Ae-kink-turn RNP switch. Nature Chemical Biology, 6(1), 71-78. https://doi.org/10.1038/nchembio.273

Synthetic translational regulation by an L7Ae-kink-turn RNP switch. / Saito, Hirohide; Kobayashi, Tetsuhiro; Hara, Tomoaki; Fujita, Yoshihiko; Hayashi, Karin; Furushima, Rie; Inoue, Tan.

In: Nature Chemical Biology, Vol. 6, No. 1, 01.2010, p. 71-78.

Research output: Contribution to journalArticle

Saito, H, Kobayashi, T, Hara, T, Fujita, Y, Hayashi, K, Furushima, R & Inoue, T 2010, 'Synthetic translational regulation by an L7Ae-kink-turn RNP switch', Nature Chemical Biology, vol. 6, no. 1, pp. 71-78. https://doi.org/10.1038/nchembio.273
Saito H, Kobayashi T, Hara T, Fujita Y, Hayashi K, Furushima R et al. Synthetic translational regulation by an L7Ae-kink-turn RNP switch. Nature Chemical Biology. 2010 Jan;6(1):71-78. https://doi.org/10.1038/nchembio.273
Saito, Hirohide ; Kobayashi, Tetsuhiro ; Hara, Tomoaki ; Fujita, Yoshihiko ; Hayashi, Karin ; Furushima, Rie ; Inoue, Tan. / Synthetic translational regulation by an L7Ae-kink-turn RNP switch. In: Nature Chemical Biology. 2010 ; Vol. 6, No. 1. pp. 71-78.
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