Synthetic terrein inhibits progression of head and neck cancer by suppressing angiogenin production

Akane Shibata, Soichiro Ibaragi, Hiroki Mandai, Toki Tsumura, Koji Kishimoto, Tatsuo Okui, Nur Mohammad Monsur Hassan, Tsuyoshi Shimo, Kazuhiro Omori, Guo Fu Hu, Shogo Takashiba, Seiji Suga, Akira Sasaki

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background/Aim: Head and neck cancers are the fifth most common cancer type worldwide, affecting more than half a million patients annually. Development of effective therapeutic drugs is, therefore, required for this type of disease. This study assessed the effects of synthetic terrein on head and neck cancer. Materials and Methods: Synthetic terrein was prepared by using the modified Altenhach's procedure. The effect of synthetic terrein on cell proliferation of head and neck cancer cells and HUVECs was assessed. Angiogenin secretion and ribosome biogenesis were examined by ELISA and silver staining of the nucleolar organizer region. A mouse xenograft model was prepared by inoculating mice with suspensions of cells of the human head and neck cancer cell line OSC-19 subcutaneously into the dorsal region of each mouse. Ki-67, CD31 and angiogenin expression in xenografted tumors was examined by immunohistochemistry. Results: Synthetic terrein inhibited the growth of various head and neck cancer cells. In addition, an in vivo experiment revealed that synthetic terrein inhibited a xenograft tumor growth in athymic mice. Immunohistochemical analysis revealed that expression of Ki-67, CD31 and ANG was down-regulated in synthetic terrein-treated tumors, compared to controls. Synthetic terrein suppressed the ANG secretion and ribosome biogenesis in cancer cells, and cell proliferation in vascular endothelial cells. Conclusion: The mechanism underlying the anti-tumor effects of synthetic terrein against head and neck cancer consists of the inhibition of both tumor cell proliferation and angiogenesis via the suppression of ANG production.

Original languageEnglish
Pages (from-to)2161-2168
Number of pages8
JournalAnticancer Research
Volume36
Issue number5
Publication statusPublished - 2016

Fingerprint

Head and Neck Neoplasms
Neoplasms
Cell Proliferation
Ribosomes
Heterografts
Nucleolus Organizer Region
Silver Staining
terrein
angiogenin
Human Umbilical Vein Endothelial Cells
Growth
Nude Mice
Suspensions
Endothelial Cells
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry
Cell Line
Pharmaceutical Preparations

Keywords

  • Angiogenesis
  • Cell proliferation
  • Head and neck cancer
  • Squamous cell carcinoma
  • Synthetic terrein

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Synthetic terrein inhibits progression of head and neck cancer by suppressing angiogenin production. / Shibata, Akane; Ibaragi, Soichiro; Mandai, Hiroki; Tsumura, Toki; Kishimoto, Koji; Okui, Tatsuo; Hassan, Nur Mohammad Monsur; Shimo, Tsuyoshi; Omori, Kazuhiro; Hu, Guo Fu; Takashiba, Shogo; Suga, Seiji; Sasaki, Akira.

In: Anticancer Research, Vol. 36, No. 5, 2016, p. 2161-2168.

Research output: Contribution to journalArticle

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abstract = "Background/Aim: Head and neck cancers are the fifth most common cancer type worldwide, affecting more than half a million patients annually. Development of effective therapeutic drugs is, therefore, required for this type of disease. This study assessed the effects of synthetic terrein on head and neck cancer. Materials and Methods: Synthetic terrein was prepared by using the modified Altenhach's procedure. The effect of synthetic terrein on cell proliferation of head and neck cancer cells and HUVECs was assessed. Angiogenin secretion and ribosome biogenesis were examined by ELISA and silver staining of the nucleolar organizer region. A mouse xenograft model was prepared by inoculating mice with suspensions of cells of the human head and neck cancer cell line OSC-19 subcutaneously into the dorsal region of each mouse. Ki-67, CD31 and angiogenin expression in xenografted tumors was examined by immunohistochemistry. Results: Synthetic terrein inhibited the growth of various head and neck cancer cells. In addition, an in vivo experiment revealed that synthetic terrein inhibited a xenograft tumor growth in athymic mice. Immunohistochemical analysis revealed that expression of Ki-67, CD31 and ANG was down-regulated in synthetic terrein-treated tumors, compared to controls. Synthetic terrein suppressed the ANG secretion and ribosome biogenesis in cancer cells, and cell proliferation in vascular endothelial cells. Conclusion: The mechanism underlying the anti-tumor effects of synthetic terrein against head and neck cancer consists of the inhibition of both tumor cell proliferation and angiogenesis via the suppression of ANG production.",
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AU - Shibata, Akane

AU - Ibaragi, Soichiro

AU - Mandai, Hiroki

AU - Tsumura, Toki

AU - Kishimoto, Koji

AU - Okui, Tatsuo

AU - Hassan, Nur Mohammad Monsur

AU - Shimo, Tsuyoshi

AU - Omori, Kazuhiro

AU - Hu, Guo Fu

AU - Takashiba, Shogo

AU - Suga, Seiji

AU - Sasaki, Akira

PY - 2016

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N2 - Background/Aim: Head and neck cancers are the fifth most common cancer type worldwide, affecting more than half a million patients annually. Development of effective therapeutic drugs is, therefore, required for this type of disease. This study assessed the effects of synthetic terrein on head and neck cancer. Materials and Methods: Synthetic terrein was prepared by using the modified Altenhach's procedure. The effect of synthetic terrein on cell proliferation of head and neck cancer cells and HUVECs was assessed. Angiogenin secretion and ribosome biogenesis were examined by ELISA and silver staining of the nucleolar organizer region. A mouse xenograft model was prepared by inoculating mice with suspensions of cells of the human head and neck cancer cell line OSC-19 subcutaneously into the dorsal region of each mouse. Ki-67, CD31 and angiogenin expression in xenografted tumors was examined by immunohistochemistry. Results: Synthetic terrein inhibited the growth of various head and neck cancer cells. In addition, an in vivo experiment revealed that synthetic terrein inhibited a xenograft tumor growth in athymic mice. Immunohistochemical analysis revealed that expression of Ki-67, CD31 and ANG was down-regulated in synthetic terrein-treated tumors, compared to controls. Synthetic terrein suppressed the ANG secretion and ribosome biogenesis in cancer cells, and cell proliferation in vascular endothelial cells. Conclusion: The mechanism underlying the anti-tumor effects of synthetic terrein against head and neck cancer consists of the inhibition of both tumor cell proliferation and angiogenesis via the suppression of ANG production.

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