Synthesis of l-cystathionine by the γ-replacement reaction of cystathionine γ-lyase from Streptomyces phaeochromogenes

Hideaki Yamada, Hiroshi Kanzaki, Toru Nagasawa

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Cystathionine γ-lyase (EC 4.4.1.1) occurring in Streptomyces phaeochromogenes (IFO 3105) can catalyze not only the α,γ-elimination reaction of l-homoserine to form α-ketobutyrate and ammonia but also the γ-replacement reaction of l-homoserine in the presence of l-cysteine to form l-cystathionine. Optimal reaction conditions for the enzymatic synthesis of l-cystathionine using the γ-replacement reaction were defined. Since concentrations of greater than 10 mM l-cysteine inhibit the γ-replacement reaction, it is necessary to keep the concentration of l-cysteine around 10 mM for a high yield of l-cystathionine. Under optimal reaction conditions, defined as the conditions producing the highest yield, 1.39 mmol of lcystathionine (0.3 g) was synthesized enzymatically from 20 mmol of l-homoserine and 5.8 mmol of l-cysteine in 100 ml of reaction mixture.

Original languageEnglish
Pages (from-to)205-217
Number of pages13
JournalJournal of biotechnology
Volume1
Issue number3-4
DOIs
Publication statusPublished - Nov 1984
Externally publishedYes

Keywords

  • Streptomyces phaeochromogenes
  • cystathionine γ-lyase
  • l-cystathionine
  • pyridoxal-phosphate dependent enzyme
  • γ-replacement reaction

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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