TY - JOUR
T1 - Synthesis of l-cystathionine by the γ-replacement reaction of cystathionine γ-lyase from Streptomyces phaeochromogenes
AU - Yamada, Hideaki
AU - Kanzaki, Hiroshi
AU - Nagasawa, Toru
N1 - Funding Information:
The authors thank Mr. Y. Koizumi for his technical assistance of measurement of the 60 MHz ‘H-NMR spectrum. This work was supported in part by Grant-in Aid for Science Research from the Ministry of Education, Science and Culture of Japan.
PY - 1984/11
Y1 - 1984/11
N2 - Cystathionine γ-lyase (EC 4.4.1.1) occurring in Streptomyces phaeochromogenes (IFO 3105) can catalyze not only the α,γ-elimination reaction of l-homoserine to form α-ketobutyrate and ammonia but also the γ-replacement reaction of l-homoserine in the presence of l-cysteine to form l-cystathionine. Optimal reaction conditions for the enzymatic synthesis of l-cystathionine using the γ-replacement reaction were defined. Since concentrations of greater than 10 mM l-cysteine inhibit the γ-replacement reaction, it is necessary to keep the concentration of l-cysteine around 10 mM for a high yield of l-cystathionine. Under optimal reaction conditions, defined as the conditions producing the highest yield, 1.39 mmol of lcystathionine (0.3 g) was synthesized enzymatically from 20 mmol of l-homoserine and 5.8 mmol of l-cysteine in 100 ml of reaction mixture.
AB - Cystathionine γ-lyase (EC 4.4.1.1) occurring in Streptomyces phaeochromogenes (IFO 3105) can catalyze not only the α,γ-elimination reaction of l-homoserine to form α-ketobutyrate and ammonia but also the γ-replacement reaction of l-homoserine in the presence of l-cysteine to form l-cystathionine. Optimal reaction conditions for the enzymatic synthesis of l-cystathionine using the γ-replacement reaction were defined. Since concentrations of greater than 10 mM l-cysteine inhibit the γ-replacement reaction, it is necessary to keep the concentration of l-cysteine around 10 mM for a high yield of l-cystathionine. Under optimal reaction conditions, defined as the conditions producing the highest yield, 1.39 mmol of lcystathionine (0.3 g) was synthesized enzymatically from 20 mmol of l-homoserine and 5.8 mmol of l-cysteine in 100 ml of reaction mixture.
KW - Streptomyces phaeochromogenes
KW - cystathionine γ-lyase
KW - l-cystathionine
KW - pyridoxal-phosphate dependent enzyme
KW - γ-replacement reaction
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U2 - 10.1016/0168-1656(84)90006-3
DO - 10.1016/0168-1656(84)90006-3
M3 - Article
AN - SCOPUS:0021741610
VL - 1
SP - 205
EP - 217
JO - Journal of Biotechnology
JF - Journal of Biotechnology
SN - 0168-1656
IS - 3-4
ER -