Synthesis of hepatitis B virus e antigen in E. coli

Takashi Inada, Yuko Misumi, Masaharu Seno, Shuichi Kanezaki, Yasuo Shibata, Yushi Oka, Haruo Onda

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7 Citations (Scopus)

Abstract

Hepatitis B virus core antigen (HBcAg) gene was deleted at some unique restriction enzyme sites, or at random, and inserted into the expression plasmids of E. coli which had the tryptophan promoter. E. coli transformants with the plasmids, synthesized materials with many kinds of antigenicity of HBcAg, HBeAg, or both HBcAg and HBeAg. HBeAg-specific material smaller than native HBeAg was produced in a stable condition.

Original languageEnglish
Pages (from-to)27-47
Number of pages21
JournalVirus research
Volume14
Issue number1
DOIs
Publication statusPublished - Sep 1989

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Keywords

  • E. coli
  • Gene expression
  • HBcAg and HBeAg
  • HBeAg specific polypeptide
  • Hepatitis B virus

ASJC Scopus subject areas

  • Cancer Research
  • Virology
  • Infectious Diseases

Cite this

Inada, T., Misumi, Y., Seno, M., Kanezaki, S., Shibata, Y., Oka, Y., & Onda, H. (1989). Synthesis of hepatitis B virus e antigen in E. coli. Virus research, 14(1), 27-47. https://doi.org/10.1016/0168-1702(89)90067-1