Synthesis of glyceryl ferulate by immobilized ferulic acid esterase

Takemasa Matsuo, Takashi Kobayashi, Yukitaka Kimura, Moriyasu Tsuchiyama, Tadanobu Oh, Tatsuji Sakamoto, Shuji Adachi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Glyceryl ferulate was synthesized by the condensation of ferulic acid with glycerol using Pectinase PL "Amano" from Aspergillus niger, which contained ferulic acid esterase, to improve the water-solubility of ferulic acid. The optimum reaction medium was glycerol/0.1 M acetate buffer, pH 4.0, (98:2 v/v). The enzyme immobilized onto Chitopearl BCW3003 exhibited the highest activity among the those immobilized onto various kinds of Chitopearl BCW resins. The optimum temperature for the immobilized enzyme was 50°C, and it could be reused at least five times without a significant loss in activity for the synthesis of glyceryl ferulate in batch reaction. Storage of the reaction mixture at 25°C improved the molar fraction of glyceryl ferulate relative to the dissolved ferulic residues.

Original languageEnglish
Pages (from-to)2151-2156
Number of pages6
JournalBiotechnology Letters
Volume30
Issue number12
DOIs
Publication statusPublished - Dec 1 2008
Externally publishedYes

Keywords

  • Ferulic acid
  • Ferulic acid esterase
  • Glyceryl ferulate
  • Immobilized enzyme
  • Repeated batch reaction

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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  • Cite this

    Matsuo, T., Kobayashi, T., Kimura, Y., Tsuchiyama, M., Oh, T., Sakamoto, T., & Adachi, S. (2008). Synthesis of glyceryl ferulate by immobilized ferulic acid esterase. Biotechnology Letters, 30(12), 2151-2156. https://doi.org/10.1007/s10529-008-9814-2