Synthesis of fluoroacetate from fluoride, glycerol, and β- hydroxypyruvate by Streptomyces cattleya

T. Tamura, M. Wada, N. Esaki, K. Soda

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Streptomyces cattleya produces fluoroacetate and 4-fluorothreonine from inorganic fluoride added to the culture broth. We have shown by 19F nuclear magnetic resonance (NMR) spectrometry that fluoroacetate is accumulated first in the culture broth and that accumulation of 4-fluorothreonine is next. To show precursors of the carbon skeleton of fluoroacetate, we carried out tracer experiments with various 14C- and 13C-labeled compounds. Radioactivity of [U-14C]glucose, [U-14C]glycerol, [U-14C]serine, and [U-14C]β-hydroxypyruvate was incorporated into fluoroacetate to an extent of 0.2 to 0.4%, whereas [3-14C]pyruvate, [2,3-14C]succinate, and [U- 14C]aspartate were less efficiently incorporated (0.04 to 0.08%). The addition of [2-13C]glycerol to the mycelium suspension of Streptomyces cattleya caused exclusive enrichment of the carboxyl carbon of fluoroacetate with 13C; about 40% of carboxyl carbon of fluoroacetate was labeled with 13C. We studied the radioactivity incorporation of [3-14C]-, [U-14C]-, and [1-14C]β-hydroxypyruvates to show that C-2 and C-3 of β-hydroxy pyruvate are exclusively converted to the carbon skeleton of fluoroacetate. These results suggest that the carbon skeleton of fluoroacetate derives from C-1 and C-2 of glycerol through β-hydroxypyruvate, whose hydroxyl group is eventually replaced by fluoride.

Original languageEnglish
Pages (from-to)2265-2269
Number of pages5
JournalJournal of bacteriology
Volume177
Issue number9
DOIs
Publication statusPublished - Jan 1 1995
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Fingerprint Dive into the research topics of 'Synthesis of fluoroacetate from fluoride, glycerol, and β- hydroxypyruvate by Streptomyces cattleya'. Together they form a unique fingerprint.

  • Cite this