TY - JOUR
T1 - Synthesis of Europium(III) Complex-Based Hydroxyapatite Nanocrystals for Biolabeling Applications
AU - Kataoka, Takuya
AU - Abe, Shigeaki
AU - Tagaya, Motohiro
N1 - Funding Information:
This study was supported by a grant from the Japan Society for the Promotion of Science (JSPS) KAKENHI (Grant-in-Aid for Young Scientists (A), Grant No. 17H04954, and Challenging Research (Exploratory), Grant No. 17K19027). The authors thank Analysis and Instrumentation Center in Nagaoka University of Technology for providing the analytical facilities.
Publisher Copyright:
© 2018 Trans Tech Publications Ltd, Switzerland.
PY - 2018
Y1 - 2018
N2 - We synthesized the inorganic/organic hybrid nanocrystal (EHA) by hydroxyapatite (HA) nanocrystal growth under the existence of tris(2,2,6,6-tetramethyl-3,5-heptanedionato)europium(III) (EuTH) complex. Then, folic acid derivative (FA-NHS: folate N-hydroxysuccinimidyl ester) as the targeting ligand for the HeLa cancer cells was immobilized on the EHA by the mediation of 3-aminopropyltriethoxysilane (APTES) and methyltriethoxysilane (MTES). When the FA-NHS molecular occupancy ratio on the EHA surface is around 3 to 5 %, the strong luminescence from the f-f transition of the Eu3+ ion and luminescence associated with energy transfer between the EuTH-FA monomer near 518 nm were observed. Moreover, the dispersibility in phosphate buffer saline was confirmed with immobilizing the positively-charged FA-NHS. The affinity and non-cytotoxicity between the nanocrystals and HeLa cancer cells were confirmed for 3 days. The red luminescence from the cells could be observed by fluorescence microscopy and the luminescence spectra.
AB - We synthesized the inorganic/organic hybrid nanocrystal (EHA) by hydroxyapatite (HA) nanocrystal growth under the existence of tris(2,2,6,6-tetramethyl-3,5-heptanedionato)europium(III) (EuTH) complex. Then, folic acid derivative (FA-NHS: folate N-hydroxysuccinimidyl ester) as the targeting ligand for the HeLa cancer cells was immobilized on the EHA by the mediation of 3-aminopropyltriethoxysilane (APTES) and methyltriethoxysilane (MTES). When the FA-NHS molecular occupancy ratio on the EHA surface is around 3 to 5 %, the strong luminescence from the f-f transition of the Eu3+ ion and luminescence associated with energy transfer between the EuTH-FA monomer near 518 nm were observed. Moreover, the dispersibility in phosphate buffer saline was confirmed with immobilizing the positively-charged FA-NHS. The affinity and non-cytotoxicity between the nanocrystals and HeLa cancer cells were confirmed for 3 days. The red luminescence from the cells could be observed by fluorescence microscopy and the luminescence spectra.
KW - Bio-labeling
KW - Europium(III) complex
KW - Exciplex emission
KW - HeLa cancer cells
KW - Hydroxyapatite
KW - Organic-inorganic nanohybrid
UR - http://www.scopus.com/inward/record.url?scp=85078784568&partnerID=8YFLogxK
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U2 - 10.4028/www.scientific.net/KEM.782.41
DO - 10.4028/www.scientific.net/KEM.782.41
M3 - Article
AN - SCOPUS:85078784568
SN - 1013-9826
VL - 782 KEM
SP - 41
EP - 46
JO - Key Engineering Materials
JF - Key Engineering Materials
ER -
