Synthesis and cytocompatibility of porous chitosan-silicate hybrids for tissue engineering scaffold application

Yuki Shirosaki, Tomoyuki Okayama, Kanji Tsuru, Satoshi Hayakawa, Akiyoshi Osaka

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Chitosan-silicate hybrids with 3D porous structures were prepared with freeze-drying precursor solutions derived from chitosan and γ-glycidoxypropyltrimethoxysilane (GPTMS). They were formed easily in any shape, such as sheets, pellets, disks, granules, and even roll-cakes. The pore size was strongly dependent on the freezing temperature: lower freezing temperature resulted smaller pores, about 110 μm for the hybrids frozen at -20 °C, and about 50 μm for those at -85 °C. The pore size was little dependent on the GPTMS content. In contrast, the GPTMS content affected porosity a littlie: ∼80% for chitosan, and ∼90% for the GPTMS-containing hybrids. Thus, their porous microstructure was controllable due to the freezing temperature and composition. MG63 osteoblastic cells were cultured up to 7 days on the porous hybrids. The cells adhered on the pore walls, proliferated, and migrated deep into the pore structure. It was thus concluded that the present chitosan-silicate hybrids were promising for tissue engineering scaffold applications.

Original languageEnglish
Pages (from-to)122-128
Number of pages7
JournalChemical Engineering Journal
Volume137
Issue number1
DOIs
Publication statusPublished - Mar 15 2008

Fingerprint

Tissue Scaffolds
Silicates
Chitosan
Scaffolds (biology)
Tissue engineering
silicate
Freezing
engineering
freezing
Pore size
freeze drying
Pore structure
Temperature
Drying
Porosity
microstructure
Microstructure
temperature
porosity
tissue

Keywords

  • γ-Glycidoxypropyltrimethoxysilane (GPTMS)
  • Chitosan
  • Cytocompatibility
  • Organic-inorganic hybrid
  • Osteoblastic cell MG63
  • Scaffold

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Environmental Engineering

Cite this

Synthesis and cytocompatibility of porous chitosan-silicate hybrids for tissue engineering scaffold application. / Shirosaki, Yuki; Okayama, Tomoyuki; Tsuru, Kanji; Hayakawa, Satoshi; Osaka, Akiyoshi.

In: Chemical Engineering Journal, Vol. 137, No. 1, 15.03.2008, p. 122-128.

Research output: Contribution to journalArticle

Shirosaki, Yuki ; Okayama, Tomoyuki ; Tsuru, Kanji ; Hayakawa, Satoshi ; Osaka, Akiyoshi. / Synthesis and cytocompatibility of porous chitosan-silicate hybrids for tissue engineering scaffold application. In: Chemical Engineering Journal. 2008 ; Vol. 137, No. 1. pp. 122-128.
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