Synthesis and chemiluminescent activity of pyridazino[4,5-b]indole-1,4(2H,3H)-diones

Substituted and non-substituted anilines were transformed to the corresponding triazole derivatives, which were converted to dimethyl indole-2,3-dicarboxylates by photocyclization. The reaction of diester with hydrazine hydrate gave 1,2,3,4-tetrahydro-5H-pyridazino[4,5-b]indole-1,4(2H,3H)-diones (5). Some of compounds (5) were found to have chemiluminescent activity in a similarly to luminol in the presence of hydrogen peroxide and peroxidase in a basic solution.