Abstract
We synthesized the selenium analog of glutathione disulfide by a liquid phase method and named it glutaselenone (i.e., γ-L-glutamyl-L-selenocysteinylglycine) diselenide. The selenol of selenocysteine was protected by the p-methoxybenzyl group, which was removed by acidolysis with trifluoroacetic acid in the presence of thioanisol. The overall yield of the final product, glutaselenone diselenide, was about 9% based on the starting compound, Se-(p-methoxybenzyl)-L-selenocysteine. Glutaselenone diselenide showed a broad absorption band between 270 and 400 nm and circular dichroism bands around 270 nm (positive) and 330 nm (negative), which were attributable to diselenide bond.
| Original language | English |
|---|---|
| Pages (from-to) | 151-154 |
| Number of pages | 4 |
| Journal | Analytical Biochemistry |
| Volume | 208 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 1993 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Molecular Biology
- Biophysics
- Biochemistry
Cite this
Synthesis and Characterization of the Selenium Analog of Glutathione Disulfide. / Tamura, Takashi; Oikawa, T.; Ohtaka, A.; Fujii, N.; Esaki, N.; Soda, K.
In: Analytical Biochemistry, Vol. 208, No. 1, 01.1993, p. 151-154.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Synthesis and Characterization of the Selenium Analog of Glutathione Disulfide
AU - Tamura, Takashi
AU - Oikawa, T.
AU - Ohtaka, A.
AU - Fujii, N.
AU - Esaki, N.
AU - Soda, K.
PY - 1993/1
Y1 - 1993/1
N2 - We synthesized the selenium analog of glutathione disulfide by a liquid phase method and named it glutaselenone (i.e., γ-L-glutamyl-L-selenocysteinylglycine) diselenide. The selenol of selenocysteine was protected by the p-methoxybenzyl group, which was removed by acidolysis with trifluoroacetic acid in the presence of thioanisol. The overall yield of the final product, glutaselenone diselenide, was about 9% based on the starting compound, Se-(p-methoxybenzyl)-L-selenocysteine. Glutaselenone diselenide showed a broad absorption band between 270 and 400 nm and circular dichroism bands around 270 nm (positive) and 330 nm (negative), which were attributable to diselenide bond.
AB - We synthesized the selenium analog of glutathione disulfide by a liquid phase method and named it glutaselenone (i.e., γ-L-glutamyl-L-selenocysteinylglycine) diselenide. The selenol of selenocysteine was protected by the p-methoxybenzyl group, which was removed by acidolysis with trifluoroacetic acid in the presence of thioanisol. The overall yield of the final product, glutaselenone diselenide, was about 9% based on the starting compound, Se-(p-methoxybenzyl)-L-selenocysteine. Glutaselenone diselenide showed a broad absorption band between 270 and 400 nm and circular dichroism bands around 270 nm (positive) and 330 nm (negative), which were attributable to diselenide bond.
UR - http://www.scopus.com/inward/record.url?scp=0027450708&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027450708&partnerID=8YFLogxK
U2 - 10.1006/abio.1993.1021
DO - 10.1006/abio.1993.1021
M3 - Article
C2 - 8434784
AN - SCOPUS:0027450708
VL - 208
SP - 151
EP - 154
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 1
ER -