Synthesis and biological characterization of monocyte-derived neutrophil chemotactic factor

Shuji Tanaka, Elizabeth A. Robinson, Teizo Yoshimura, Kouji Matsushima, Edward J. Leonard, Ettore Appella

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

MDNCF is a human monocyte-derived, 72-residue chemotactic peptide, which has sequence similarity with members of a family of pro-inflammatory cytokines. The peptide was synthesized by the solid-phase method, and is identical to the natural peptide in amino acid composition, sequence and chemotactic potency. MDNCF forms two loops via a neighboring pair of disulfide bridges, the probable locations of which are residues 7-34 and 9-50. Reduction and alkylation eliminated chemotactic activity. MDNCF fragments 7-37, 30-72 and 17-72 were all biologically inactive. The data suggest that the region of the clustered pair of disulfide bridges is important for biological activity.

Original languageEnglish
Pages (from-to)467-470
Number of pages4
JournalFEBS Letters
Volume236
Issue number2
DOIs
Publication statusPublished - Aug 29 1988
Externally publishedYes

Fingerprint

Interleukin-8
Disulfides
Peptides
Alkylation
Bioactivity
Monocytes
Amino Acid Sequence
Thermodynamic properties
Cytokines
Amino Acids
Chemical analysis

Keywords

  • Neutrophil chemotaxis
  • Peptide synthesis

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Synthesis and biological characterization of monocyte-derived neutrophil chemotactic factor. / Tanaka, Shuji; Robinson, Elizabeth A.; Yoshimura, Teizo; Matsushima, Kouji; Leonard, Edward J.; Appella, Ettore.

In: FEBS Letters, Vol. 236, No. 2, 29.08.1988, p. 467-470.

Research output: Contribution to journalArticle

Tanaka, Shuji ; Robinson, Elizabeth A. ; Yoshimura, Teizo ; Matsushima, Kouji ; Leonard, Edward J. ; Appella, Ettore. / Synthesis and biological characterization of monocyte-derived neutrophil chemotactic factor. In: FEBS Letters. 1988 ; Vol. 236, No. 2. pp. 467-470.
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