Synthesis and biological characterization of monocyte-derived neutrophil chemotactic factor

Shuji Tanaka, Elizabeth A. Robinson, Teizo Yoshimura, Kouji Matsushima, Edward J. Leonard, Ettore Appella

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

MDNCF is a human monocyte-derived, 72-residue chemotactic peptide, which has sequence similarity with members of a family of pro-inflammatory cytokines. The peptide was synthesized by the solid-phase method, and is identical to the natural peptide in amino acid composition, sequence and chemotactic potency. MDNCF forms two loops via a neighboring pair of disulfide bridges, the probable locations of which are residues 7-34 and 9-50. Reduction and alkylation eliminated chemotactic activity. MDNCF fragments 7-37, 30-72 and 17-72 were all biologically inactive. The data suggest that the region of the clustered pair of disulfide bridges is important for biological activity.

Original languageEnglish
Pages (from-to)467-470
Number of pages4
JournalFEBS Letters
Volume236
Issue number2
DOIs
Publication statusPublished - Aug 29 1988
Externally publishedYes

Keywords

  • Neutrophil chemotaxis
  • Peptide synthesis

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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