TY - JOUR
T1 - Syntheses of S-Substituted L-Homocysteine Derivatives by Cystathionine γ-Lyase of Streptomyces phaeochromogenes
AU - Kanzaki, Hiroshi
AU - Kobayashi, Michihiko
AU - Nagasawa, Toru
AU - Yamada, Hideaki
N1 - Funding Information:
This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.
PY - 1986
Y1 - 1986
N2 - Cystathionine y-lyase from Streptomyces phaeochromogenes catalyzes not only the α,γ-elimination reaction of L-cystathionine, but also the y-replacement reaction of L-homoserine in the presence of thiol compounds. Substrates for the enzyme in the y-replacement reaction were examined. It was found that D-cysteine, L- and D-homocysteine, and 3- and 2-mercaptopropionate served as preferable substrates in the y-replacement reaction. D-Allocystathionine,*1L- and mesohomolanthionine, S-carboxyethyl-L-homocysteine and S-methylcarboxymethyl-L-homocysteine were enzymatically synthesized from L-homoserine and the corresponding thiol compounds. The thus synthesized S-substituted L-homocysteine derivatives were isolated from large scale reaction mixtures and identified physicochemically.
AB - Cystathionine y-lyase from Streptomyces phaeochromogenes catalyzes not only the α,γ-elimination reaction of L-cystathionine, but also the y-replacement reaction of L-homoserine in the presence of thiol compounds. Substrates for the enzyme in the y-replacement reaction were examined. It was found that D-cysteine, L- and D-homocysteine, and 3- and 2-mercaptopropionate served as preferable substrates in the y-replacement reaction. D-Allocystathionine,*1L- and mesohomolanthionine, S-carboxyethyl-L-homocysteine and S-methylcarboxymethyl-L-homocysteine were enzymatically synthesized from L-homoserine and the corresponding thiol compounds. The thus synthesized S-substituted L-homocysteine derivatives were isolated from large scale reaction mixtures and identified physicochemically.
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U2 - 10.1271/bbb1961.50.391
DO - 10.1271/bbb1961.50.391
M3 - Article
AN - SCOPUS:85004415594
VL - 50
SP - 391
EP - 397
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 2
ER -