Syntheses of s-substituted l-homocysteine derivatives by cystathionine γ-lyase of streptomyces phaeochromogenes

Hiroshi Kanzaki, Michihiko Kobayashi, Torn Nagasawa, Hideaki Yamada

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Cystathionine γ-lyase from Streptomyces phaeochromogenes catalyzes not only the a, y-elimination reaction of L-cystathionine, but also the γ-replacement reaction of L-homoserine in the presence of thiol compounds. Substrates for the enzyme in the y-replacement reaction were examined. It was found that D-cysteine, land D-homocysteine, and 3-and 2-mercaptopropionate served as preferable substrates in the y-replacement reaction, d-Allocystathionine, *1 L-and meso-homolanthionine, S-carboxyethyl-L-homocysteine and S'-methylcarboxymethyl-L-homocysteine were enzymatically synthesized from L-homoserine and the corresponding thiol compounds. The thus synthesized S-substituted L-homocysteine derivatives were isolated from large scale reaction mixtures and identified physicochemically.

Original languageEnglish
Pages (from-to)391-397
Number of pages7
JournalAgricultural and Biological Chemistry
Volume50
Issue number2
DOIs
Publication statusPublished - Feb 1986
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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