Synergistic inhibition of growth by aluminum and iron of tobacco (Nicotiana tabacum L.) cells in suspension culture

Kanji Ono, Yoko Yamamoto, Akiko Hachiya, Hideaki Matsumoto

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Growth of tobacco cells at the logarithmic phase was inhibited after treatment with both AlCl3 and FeSO4 in modified Murashige-Skoog medium prepared without Pi and EDTA at pH 5.0 for up to 20 h, whereas cells treated with either AlCl3 or FeSO4 (at concentrations up to 100 μM, respectively) grew normally. These results suggest the synergistic inhibition of growth by Al3+ and Fe2+ ions. During the exposure to both salts, there was a lag time of about 10 h before growth inhibition became apparent in cultures of cells upon treatment with the two salts together. Then the extent of inhibition increased and reached a maximum value after exposure for 18 h. After the lag period, cells treated with both salts, but not cells treated with either salt alone, exhibited significant increases in the amounts of both aluminum and iron in the cells and could be stained with hematoxylin, with the nuclei staining especially strongly. Cells treated with both salts also exhibited a decrease in the number of cells that could be plasmolyzed in 1 M mannitol, a decrease in the number of cells that could be stained with fluorescein diacetate, a decrease in the amount of potassium in cells, and an increase in the extent of lipid peroxidation. These results suggest that aluminum causes the peroxidation of lipids in the presence of iron and that lipid peroxidation alters the permeability of the plasma membrane and leads to cell death.

Original languageEnglish
Pages (from-to)115-125
Number of pages11
JournalPlant and Cell Physiology
Volume36
Issue number1
Publication statusPublished - Jan 1995

Fingerprint

Nicotiana Tabacum
Suspension Culture
Tobacco
Aluminum
tobacco
Iron
Nicotiana tabacum
growth retardation
cell suspension culture
aluminum
Suspensions
iron
Salts
salt
Lipids
Cell
Growth
lipid
Salt
Lipid Peroxidation

Keywords

  • Aluminum
  • Iron
  • Lipid peroxidation
  • Membrane permeability
  • Synergistic effect
  • Tobacco (Nicotiana tabacum)

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Ecology
  • Cell Biology
  • Physiology
  • Plant Science

Cite this

Synergistic inhibition of growth by aluminum and iron of tobacco (Nicotiana tabacum L.) cells in suspension culture. / Ono, Kanji; Yamamoto, Yoko; Hachiya, Akiko; Matsumoto, Hideaki.

In: Plant and Cell Physiology, Vol. 36, No. 1, 01.1995, p. 115-125.

Research output: Contribution to journalArticle

Ono, Kanji ; Yamamoto, Yoko ; Hachiya, Akiko ; Matsumoto, Hideaki. / Synergistic inhibition of growth by aluminum and iron of tobacco (Nicotiana tabacum L.) cells in suspension culture. In: Plant and Cell Physiology. 1995 ; Vol. 36, No. 1. pp. 115-125.
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