Synergistic effect of [10]-gingerol and aminoglycosides against vancomycin-resistant enterococci (VRE)

Chihiro Nagoshi, Sumiko Shiota, Teruo Kuroda, Tsutomu Hatano, Takashi Yoshida, Reiko Kariyama, Tomofusa Tsuchiya

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

An extract from ginger (root of Zingiber officinale) reduced the minimum inhibitory concentrations (MICs) of aminoglycosides in vancomycin-resistant enterococci (VRE). The effective compound was isolated and identified as [10]-gingerol. In the presence of [10]-gingerol at 1/10 concentration of its own MIC, the MIC of arbekacin was lowered by 1/32 to 1/16. [10]-Gingerol also reduced the MICs of other aminoglycosides, and of bacitracin and polymixin B, but not of other antimicrobial agents tested. Because [10]-gingerol reduced the MICs of several aminoglycosides both in strains possessing or lacking aminoglycoside-modification enzymes, it seems that the effect of [10]-gingerol is not related to these enzymes, which mainly confer bacterial resistance against aminoglycosides. It seemed that a detergent-like effect of [10]-gingerol potentiated the antimicrobial activity of the aminoglycosides. In fact, some detergents such as sodium dodecyl sulfate (SDS) and Triton X-100 reduced the MICs of aminoglycosides, bacitracin and polymixin B in VRE. Since the intrinsic resistance to aminoglycosides in enterococci is due to low level of entry of the drugs into the cells, increase in the membrane permeability caused by [10]-gingerol will enhance the influx of aminoglycosides into enterococcal cells.

Original languageEnglish
Pages (from-to)443-447
Number of pages5
JournalBiological and Pharmaceutical Bulletin
Volume29
Issue number3
DOIs
Publication statusPublished - Mar 2006

Fingerprint

Aminoglycosides
Microbial Sensitivity Tests
Ginger
Detergents
Vancomycin-Resistant Enterococci
gingerol
Enterococcus
Octoxynol
Enzymes
Anti-Infective Agents
Sodium Dodecyl Sulfate
Permeability
Membranes

Keywords

  • [10]-gingerol
  • Aminoglycoside
  • Arbekacin
  • Vancomycin-resistant enterococci (VRE)

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Synergistic effect of [10]-gingerol and aminoglycosides against vancomycin-resistant enterococci (VRE). / Nagoshi, Chihiro; Shiota, Sumiko; Kuroda, Teruo; Hatano, Tsutomu; Yoshida, Takashi; Kariyama, Reiko; Tsuchiya, Tomofusa.

In: Biological and Pharmaceutical Bulletin, Vol. 29, No. 3, 03.2006, p. 443-447.

Research output: Contribution to journalArticle

Nagoshi, Chihiro ; Shiota, Sumiko ; Kuroda, Teruo ; Hatano, Tsutomu ; Yoshida, Takashi ; Kariyama, Reiko ; Tsuchiya, Tomofusa. / Synergistic effect of [10]-gingerol and aminoglycosides against vancomycin-resistant enterococci (VRE). In: Biological and Pharmaceutical Bulletin. 2006 ; Vol. 29, No. 3. pp. 443-447.
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