Synergistic bacteriolysis by bacteriophage φeF24C endolysin ORF9 and lantibiotic nisin and its application to pulsed-field gel electrophoretic analysis of Enterococcus faecalis

Iyo Takemura-Uchiyama, Jumpei Uchiyama, Miho Satoh, Takako Ujihara, Masanori Daibata, Shigenobu Matsuzaki

Research output: Contribution to journalArticlepeer-review

Abstract

Genotyping by pulsed-field gel electrophoresis (PFGE) is recognized as one of the most useful methods for epidemiological studies of drug-resistant Enterococcus faecalis nosocomial outbreaks. As the bacteriolysis procedure is a critical step in PFGE, an accurate and reliable alternative bacteriolytic method to the conventional protocol would be useful. In this study, we examined the applicability of endolysin ORF9, derived from the E. faecalis phage φEF24C, and lantibiotic nisin in sample preparation for PFGE. The results show that the cooperative actions of nisin and ORF9 synergistically lysed E. faecalis, which was then amenable to PFGE analysis.

Original languageEnglish
Pages (from-to)1209-1211
Number of pages3
JournalAnnals of Microbiology
Volume63
Issue number3
DOIs
Publication statusPublished - Sep 2013
Externally publishedYes

Keywords

  • Bacteriophage
  • Endolysin
  • Enterococcus faecalis
  • Nisin
  • Pulsed-field gel electrophoresis

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

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