Synchronization of meiosis in porcine oocytes by exposure to dibutyryl cyclic adenosine monophosphate improves developmental competence following in vitro fertilization

Hiroaki Funahashi, Thomas C. Cantley, Billy N. Day

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274 Citations (Scopus)

Abstract

The effect of stage of maturation of the germinal vesicle of porcine oocytes at the time of in vitro maturation on subsequent developmental competence was examined. A large variation exists in the germinal vesicle morphology of oocytes at the time of collection of cumulus-oocyte complexes (COCs) and after culture in the absence of dibutyryl cAMP (dbcAMP) for 20 h. However, the morphology of the germinal vesicle was synchronized to a specific stage after culture in the presence of 1 mM dbcAMP for 20 h. There was no difference in germinal vesicle breakdown rate (total mean, 75.0 ± 5.4%) or in maturation rate (total mean, 82.1 ± 2.1%) at 28 and 44 h of culture, respectively. However, differences in meiotic progress of oocytes were observed (p <0.05) at 36 h of culture when COCs were exposed to dbcAMP for the first 20 h of maturation, as compared to controls. The incidence of embryos that developed to the blastocyst stage after in vitro fertilization was higher (p <0.05) when COCs were exposed to dbcAMP (21.5 ± 2.5%) as compared to controls (9.2 ± 1.6%). After transfer of experimental embryos to four recipient gilts, the three pregnant recipients delivered 19 live piglets. These results indicate that exposure of COCs to dbcAMP for the first 20 h of culture for maturation increases the homogeneity of oocyte nuclear maturation and improves the efficiency of in vitro production of swine embryos.

Original languageEnglish
Pages (from-to)49-53
Number of pages5
JournalBiology of Reproduction
Volume57
Issue number1
DOIs
Publication statusPublished - Jul 1997

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Meiosis
Fertilization in Vitro
Cyclic AMP
Mental Competency
Oocytes
Swine
Embryonic Structures
Oocyte Retrieval
Embryo Transfer
Blastocyst
Incidence

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

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title = "Synchronization of meiosis in porcine oocytes by exposure to dibutyryl cyclic adenosine monophosphate improves developmental competence following in vitro fertilization",
abstract = "The effect of stage of maturation of the germinal vesicle of porcine oocytes at the time of in vitro maturation on subsequent developmental competence was examined. A large variation exists in the germinal vesicle morphology of oocytes at the time of collection of cumulus-oocyte complexes (COCs) and after culture in the absence of dibutyryl cAMP (dbcAMP) for 20 h. However, the morphology of the germinal vesicle was synchronized to a specific stage after culture in the presence of 1 mM dbcAMP for 20 h. There was no difference in germinal vesicle breakdown rate (total mean, 75.0 ± 5.4{\%}) or in maturation rate (total mean, 82.1 ± 2.1{\%}) at 28 and 44 h of culture, respectively. However, differences in meiotic progress of oocytes were observed (p <0.05) at 36 h of culture when COCs were exposed to dbcAMP for the first 20 h of maturation, as compared to controls. The incidence of embryos that developed to the blastocyst stage after in vitro fertilization was higher (p <0.05) when COCs were exposed to dbcAMP (21.5 ± 2.5{\%}) as compared to controls (9.2 ± 1.6{\%}). After transfer of experimental embryos to four recipient gilts, the three pregnant recipients delivered 19 live piglets. These results indicate that exposure of COCs to dbcAMP for the first 20 h of culture for maturation increases the homogeneity of oocyte nuclear maturation and improves the efficiency of in vitro production of swine embryos.",
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