Synaptojanin 1: Localization on coated endocytic intermediates in nerve terminals and interaction of its 170 kDa isoform with Eps15

Christof Haffner, Kohji Takei, Hong Chen, Niels Ringstad, Amy Hudson, Margaret Husta Butler, Anna Elisabetta Salcini, Pier Paolo Di Fiore, Pietro De Camilli

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128 Citations (Scopus)

Abstract

Synaptojanin 1 is an inositol 5-phosphatase with a putative role in clathrin-mediated endocytosis. Goal of this study was to provide new evidence for this hypothesis. We show that synaptojanin 1 is concentrated at clathrin-coated endocytic intermediates in nerve terminals. Furthermore, we report that synaptojanin-170, an alternatively spliced isoform of synaptojanin 1, binds Eps15, a clathrin coat-associated protein. Binding is mediated by the COOH-terminal region of synaptojanin-170 which we show here to be poorly conserved from rat to humans, but to contain in both species three asparagine-proline-phenylalanine (NPF) repeats. This motif has been found to be the core of the binding site for the EH domains of Eps15. Together with previous data, our results suggest that synaptojanin 1 can be recruited to clathrin-eoated pits via a multiplicity of interactions.

Original languageEnglish
Pages (from-to)175-180
Number of pages6
JournalFEBS Letters
Volume419
Issue number2-3
DOIs
Publication statusPublished - Dec 15 1997
Externally publishedYes

Keywords

  • Dynamin
  • Endocytosis
  • Inositol 5-phosphatase
  • Synapse
  • Synaptic vesicle

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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    Haffner, C., Takei, K., Chen, H., Ringstad, N., Hudson, A., Butler, M. H., Salcini, A. E., Di Fiore, P. P., & De Camilli, P. (1997). Synaptojanin 1: Localization on coated endocytic intermediates in nerve terminals and interaction of its 170 kDa isoform with Eps15. FEBS Letters, 419(2-3), 175-180. https://doi.org/10.1016/S0014-5793(97)01451-8