Synaptic vesicle endocytosis impaired by disruption of dynamin-SH3 domain interactions

Oleg Shupliakov, Peter Löw, Detlev Grabs, Helge Gad, Hong Chen, Carol David, Kohji Takei, Pietro De Camilli, Lennart Brodin

Research output: Contribution to journalArticle

379 Citations (Scopus)

Abstract

The proline-rich COOH-terminal region of dynamin binds various Src homology 3 (SH3) domain-containing proteins, but the physiological role of these interactions is unknown. In living nerve terminals, the function of the interaction with SH3 domains was examined. Amphiphysin contains an SH3 domain and is a major dynamin binding partner at the synapse. Microinjection of amphiphysin's SH3 domain or of a dynamin peptide containing the SH3 binding site inhibited synaptic vesicle endocytosis at the stage of invaginated clathrin-coated pits, which resulted in an activity-dependent distortion of the synaptic architecture and a depression of transmitter release. These findings demonstrate that SH3-mediated interactions are required for dynamin function and support an essential role of clathrin-mediated endocytosis in synaptic vesicle recycling.

Original languageEnglish
Pages (from-to)259-263
Number of pages5
JournalScience
Volume276
Issue number5310
DOIs
Publication statusPublished - Apr 11 1997
Externally publishedYes

Fingerprint

Dynamins
src Homology Domains
Synaptic Vesicles
Endocytosis
Clathrin
Src peptide
Microinjections
Proline
Synapses
Binding Sites
Proteins
amphiphysin

ASJC Scopus subject areas

  • General

Cite this

Shupliakov, O., Löw, P., Grabs, D., Gad, H., Chen, H., David, C., ... Brodin, L. (1997). Synaptic vesicle endocytosis impaired by disruption of dynamin-SH3 domain interactions. Science, 276(5310), 259-263. https://doi.org/10.1126/science.276.5310.259

Synaptic vesicle endocytosis impaired by disruption of dynamin-SH3 domain interactions. / Shupliakov, Oleg; Löw, Peter; Grabs, Detlev; Gad, Helge; Chen, Hong; David, Carol; Takei, Kohji; De Camilli, Pietro; Brodin, Lennart.

In: Science, Vol. 276, No. 5310, 11.04.1997, p. 259-263.

Research output: Contribution to journalArticle

Shupliakov, O, Löw, P, Grabs, D, Gad, H, Chen, H, David, C, Takei, K, De Camilli, P & Brodin, L 1997, 'Synaptic vesicle endocytosis impaired by disruption of dynamin-SH3 domain interactions', Science, vol. 276, no. 5310, pp. 259-263. https://doi.org/10.1126/science.276.5310.259
Shupliakov O, Löw P, Grabs D, Gad H, Chen H, David C et al. Synaptic vesicle endocytosis impaired by disruption of dynamin-SH3 domain interactions. Science. 1997 Apr 11;276(5310):259-263. https://doi.org/10.1126/science.276.5310.259
Shupliakov, Oleg ; Löw, Peter ; Grabs, Detlev ; Gad, Helge ; Chen, Hong ; David, Carol ; Takei, Kohji ; De Camilli, Pietro ; Brodin, Lennart. / Synaptic vesicle endocytosis impaired by disruption of dynamin-SH3 domain interactions. In: Science. 1997 ; Vol. 276, No. 5310. pp. 259-263.
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