Sympathetic afferent stimulation inhibits central vagal activation induced by intravenous medetomidine in rats

T. Kawada, T. Akiyama, S. Shimizu, A. Kamiya, K. Uemura, M. J. Turner, M. Shirai, M. Sugimachi

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9 Citations (Scopus)

Abstract

Aim: To examine whether sympathetic afferent stimulation (SAS) inhibits central vagal activation induced by α2-adrenergic stimulation. Methods: In anaesthetized Wistar-Kyoto rats, a cardiac microdialysis technique was applied to the left ventricle, and the effect of α2-adrenergic stimulation by medetomidine on myocardial interstitial acetylcholine (ACh) levels was examined in the absence (n = 6) or the presence (n = 6) of SAS delivered from the left stellate ganglion. The effect of electrical vagal efferent stimulation on myocardial interstitial ACh release was also examined in the absence or the presence of SAS (n = 6). Results: Intravenous medetomidine (0.1 mg kg-1) significantly increased myocardial interstitial ACh levels in the absence of SAS (from 1.95 ± 0.79 to 3.36 ± 1.61 nm, P < 0.05), but not in the presence of SAS (from 1.67 ± 0.67 to 2.01 ± 0.78 nm). In contrast, electrical vagal nerve stimulation increased myocardial interstitial ACh level to the same degree regardless of SAS (from 1.66 ± 0.16 to 3.93 ± 0.72 nm without SAS vs. 4.05 ± 0.89 nm with SAS). Conclusion: Sympathetic afferent stimulation inhibited medetomidine-induced ACh release, but not electrical stimulation-induced ACh release, suggesting that SAS inhibited medetomidine-induced vagal activation via central mechanisms. While central vagal activation by α2-adrenergic agonists could be an alternative to electrical vagal activation, blocking sympathetic afferent input may be important to increase the efficacy of α2-adrenergic agonists in enhancing vagal nerve activity.

Original languageEnglish
Pages (from-to)55-61
Number of pages7
JournalActa Physiologica
Volume209
Issue number1
DOIs
Publication statusPublished - Sep 2013
Externally publishedYes

Keywords

  • Acetylcholine
  • Cardiac microdialysis
  • Medetomidine
  • Rats
  • Vagal nerve stimulation
  • Visceral afferent

ASJC Scopus subject areas

  • Physiology

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  • Cite this

    Kawada, T., Akiyama, T., Shimizu, S., Kamiya, A., Uemura, K., Turner, M. J., Shirai, M., & Sugimachi, M. (2013). Sympathetic afferent stimulation inhibits central vagal activation induced by intravenous medetomidine in rats. Acta Physiologica, 209(1), 55-61. https://doi.org/10.1111/apha.12123