Susceptibility of Human T-Lymphotropic Virus Type I-Infected Cell Line MT-2 to Hepatitis C Virus Infection

Nobuyuki Kato, T. Nakazawa, T. Mizutani, K. Shimotohno

Research output: Contribution to journalArticle

113 Citations (Scopus)

Abstract

To obtain a hepatitis C virus (HCV) proliferation system, we examined the susceptibility of various cultured cell lines to HCV infection. We found that a human T-lymphotropic virus type I infected cell Line MT-2 was fairly sensitive to HCV infection. Using the polymerase chain reaction, intracellular positive-stranded HCV RNA was detected until at least 15 days postinoculation (p.i.). Intracellular negative-stranded HCV RNA was also detected at 10 days p.i., although not at 7 days p.i., suggesting that HCV is replicating in MT-2 cells 10 days p.i. Sequence analysis of hypervariable region 1 (HVR1) revealed that HVR1 sequences from cells 10 days p.i. had become homogeneous, although HVR1 sequences from the inoculum showed the typical quasi-species. We also found a lack of anti-HVR1 antibody against the HVR1 species which became homogeneous at 10 days p.i., although we easily detected antibody against the other HVR1 species obtained from the inoculum. These findings suggest that MT-2 cells are susceptible to HCV infection and are capable of supporting HCV replication.

Original languageEnglish
Pages (from-to)863-869
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume206
Issue number3
DOIs
Publication statusPublished - Jan 26 1995
Externally publishedYes

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Virus Diseases
Viruses
Hepacivirus
Cells
Cell Line
RNA
Antibodies
Virus Replication
Polymerase chain reaction
Sequence Analysis
Cultured Cells
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology
  • Biophysics
  • Biochemistry

Cite this

Susceptibility of Human T-Lymphotropic Virus Type I-Infected Cell Line MT-2 to Hepatitis C Virus Infection. / Kato, Nobuyuki; Nakazawa, T.; Mizutani, T.; Shimotohno, K.

In: Biochemical and Biophysical Research Communications, Vol. 206, No. 3, 26.01.1995, p. 863-869.

Research output: Contribution to journalArticle

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