Susceptibility of erythrocytes from several animal species to Vibrio vulnificus hemolysin

Hiroyasu Yamanaka, Shinobu Shimatani, Midori Tanaka, Takashi Katsu, Bun ichiro Ono, Sumio Shinoda

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The hemolytic activity of Vibrio vulnificus hemolysin (VVH) against erythrocytes from several animal species (sheep, horse, cow, rabbit, chicken) was investigated. VVH was active against erythrocytes from all species, but the amount of VVH causing 50% hemolysis under identical conditions (hemolytic susceptibility to VVH) differed. The degree of 125I-labeled VVH (125I-VVH) binding to each erythrocyte species correlated with the susceptibility of the cells to hemolysis. However, marked differences in the binding ability of 125I- VVH were not observed against liposomes constructed with lipids from each erythrocyte membrane. On the other hand, release of hemoglobin (Hb) differed for each of the erythrocyte species despite administration of approximately the same hemolytic VVH concentration to each species. Furthermore, under hypotonic conditions, the stability of each erythrocyte species varied markedly; the more susceptible the erythrocyte to VVH, the more unstable it was under such conditions. These results, therefore, suggest that the susceptibility of erythrocytes to VVH may be closely associated with the binding ability of VVH and erythrocyte membrane stability.

Original languageEnglish
Pages (from-to)251-255
Number of pages5
JournalFEMS Microbiology Letters
Volume61
Issue number3
Publication statusPublished - Oct 15 1989

Fingerprint

Vibrio vulnificus
Erythrocytes
Erythrocyte Membrane
Hemolysis
Vibrio hemolysin
Liposomes
Horses
Chickens
Sheep
Hemoglobins

Keywords

  • Cytolysin
  • Erythrocyte
  • Hemolysin
  • Hemolysis
  • Vibrio
  • Vibrio vulnificus

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Yamanaka, H., Shimatani, S., Tanaka, M., Katsu, T., Ono, B. I., & Shinoda, S. (1989). Susceptibility of erythrocytes from several animal species to Vibrio vulnificus hemolysin. FEMS Microbiology Letters, 61(3), 251-255.

Susceptibility of erythrocytes from several animal species to Vibrio vulnificus hemolysin. / Yamanaka, Hiroyasu; Shimatani, Shinobu; Tanaka, Midori; Katsu, Takashi; Ono, Bun ichiro; Shinoda, Sumio.

In: FEMS Microbiology Letters, Vol. 61, No. 3, 15.10.1989, p. 251-255.

Research output: Contribution to journalArticle

Yamanaka, H, Shimatani, S, Tanaka, M, Katsu, T, Ono, BI & Shinoda, S 1989, 'Susceptibility of erythrocytes from several animal species to Vibrio vulnificus hemolysin', FEMS Microbiology Letters, vol. 61, no. 3, pp. 251-255.
Yamanaka H, Shimatani S, Tanaka M, Katsu T, Ono BI, Shinoda S. Susceptibility of erythrocytes from several animal species to Vibrio vulnificus hemolysin. FEMS Microbiology Letters. 1989 Oct 15;61(3):251-255.
Yamanaka, Hiroyasu ; Shimatani, Shinobu ; Tanaka, Midori ; Katsu, Takashi ; Ono, Bun ichiro ; Shinoda, Sumio. / Susceptibility of erythrocytes from several animal species to Vibrio vulnificus hemolysin. In: FEMS Microbiology Letters. 1989 ; Vol. 61, No. 3. pp. 251-255.
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