Survival of human dental pulp cells after 4-week culture in human tooth model

Mariano S. Pedano, Xin Li, Charlotte Jeanneau, Manosij Ghosh, Kumiko Yoshihara, Kirsten Van Landuyt, Imad About, Bart Van Meerbeek

Research output: Contribution to journalArticle

Abstract

Objectives: This study aimed to validate the human tooth model by investigating the growth efficiency, expression of mesenchymal stem cell (MSC) markers and differentiation ability of human dental pulp cells (hDPCs) harvested from extracted immature third molars and cultured for different periods. Moreover, the effect of exposure and capping with a hydraulic calcium-silicate cement on pulp tissue after 4-week culture in the tooth model was investigated. Methods: Primary hDPCs were collected from 18 molars from six individuals (15–19 years). One tooth of each patient was immediately cultured (control), while the other teeth were exposed to culture medium for 1, 2 or 4 weeks. After different culture periods, cells were harvested using the explant method, upon which cells were evaluated for cell-doubling time, colony-forming efficiency and expression of cell surface markers. The osteogenic, adipogenic and chondrogenic differentiation efficacy was also determined. Two teeth from three different patients (n = 6) were used for the pulp-capping assay. Three teeth were capped with ProRoot MTA (Dentsply Sirona), while three other exposed teeth remained uncapped (control). Results: Cells were found to grow, express MSC markers and showed osteogenic, adipogenic and chondrogenic differentiation potential at all time periods. Histology of the teeth subjected to the pulp-capping assay showed the formation of mineralized tissue after 4-week exposure to ProRoot MTA (Dentsply Sirona) and normal histological features in the control teeth. Conclusions: This study confirmed that hDPCs of teeth cultured for up to 4 weeks in a human tooth model are viable, express MSC markers and show differentiation ability. Clinical Significance: The human tooth model can be seen as an advanced cell-culture model that makes use of the original 3D pulp-chamber structure. It can serve as a screening tool to evaluate new pulp-capping formulations in a relatively cheap and fast manner.

Original languageEnglish
Pages (from-to)33-40
Number of pages8
JournalJournal of Dentistry
Volume86
DOIs
Publication statusPublished - Jul 1 2019

Fingerprint

Dental Pulp
Tooth
Dental Pulp Capping
Pemetrexed
Mesenchymal Stromal Cells
Silicate Cement
Cell Culture Techniques
Third Molar
Deciduous Tooth
Dental Pulp Cavity
Differentiation Antigens
Culture Media
Cell Differentiation
Cultured Cells
Histology

Keywords

  • Biomaterials
  • Calcium-silicate cements
  • Pulp biology
  • Pulp capping
  • Tissue engineering
  • Vital pulp therapy

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Pedano, M. S., Li, X., Jeanneau, C., Ghosh, M., Yoshihara, K., Van Landuyt, K., ... Van Meerbeek, B. (2019). Survival of human dental pulp cells after 4-week culture in human tooth model. Journal of Dentistry, 86, 33-40. https://doi.org/10.1016/j.jdent.2019.05.023

Survival of human dental pulp cells after 4-week culture in human tooth model. / Pedano, Mariano S.; Li, Xin; Jeanneau, Charlotte; Ghosh, Manosij; Yoshihara, Kumiko; Van Landuyt, Kirsten; About, Imad; Van Meerbeek, Bart.

In: Journal of Dentistry, Vol. 86, 01.07.2019, p. 33-40.

Research output: Contribution to journalArticle

Pedano, MS, Li, X, Jeanneau, C, Ghosh, M, Yoshihara, K, Van Landuyt, K, About, I & Van Meerbeek, B 2019, 'Survival of human dental pulp cells after 4-week culture in human tooth model', Journal of Dentistry, vol. 86, pp. 33-40. https://doi.org/10.1016/j.jdent.2019.05.023
Pedano, Mariano S. ; Li, Xin ; Jeanneau, Charlotte ; Ghosh, Manosij ; Yoshihara, Kumiko ; Van Landuyt, Kirsten ; About, Imad ; Van Meerbeek, Bart. / Survival of human dental pulp cells after 4-week culture in human tooth model. In: Journal of Dentistry. 2019 ; Vol. 86. pp. 33-40.
@article{29e08de22f0f452f9dc32babe08fca78,
title = "Survival of human dental pulp cells after 4-week culture in human tooth model",
abstract = "Objectives: This study aimed to validate the human tooth model by investigating the growth efficiency, expression of mesenchymal stem cell (MSC) markers and differentiation ability of human dental pulp cells (hDPCs) harvested from extracted immature third molars and cultured for different periods. Moreover, the effect of exposure and capping with a hydraulic calcium-silicate cement on pulp tissue after 4-week culture in the tooth model was investigated. Methods: Primary hDPCs were collected from 18 molars from six individuals (15–19 years). One tooth of each patient was immediately cultured (control), while the other teeth were exposed to culture medium for 1, 2 or 4 weeks. After different culture periods, cells were harvested using the explant method, upon which cells were evaluated for cell-doubling time, colony-forming efficiency and expression of cell surface markers. The osteogenic, adipogenic and chondrogenic differentiation efficacy was also determined. Two teeth from three different patients (n = 6) were used for the pulp-capping assay. Three teeth were capped with ProRoot MTA (Dentsply Sirona), while three other exposed teeth remained uncapped (control). Results: Cells were found to grow, express MSC markers and showed osteogenic, adipogenic and chondrogenic differentiation potential at all time periods. Histology of the teeth subjected to the pulp-capping assay showed the formation of mineralized tissue after 4-week exposure to ProRoot MTA (Dentsply Sirona) and normal histological features in the control teeth. Conclusions: This study confirmed that hDPCs of teeth cultured for up to 4 weeks in a human tooth model are viable, express MSC markers and show differentiation ability. Clinical Significance: The human tooth model can be seen as an advanced cell-culture model that makes use of the original 3D pulp-chamber structure. It can serve as a screening tool to evaluate new pulp-capping formulations in a relatively cheap and fast manner.",
keywords = "Biomaterials, Calcium-silicate cements, Pulp biology, Pulp capping, Tissue engineering, Vital pulp therapy",
author = "Pedano, {Mariano S.} and Xin Li and Charlotte Jeanneau and Manosij Ghosh and Kumiko Yoshihara and {Van Landuyt}, Kirsten and Imad About and {Van Meerbeek}, Bart",
year = "2019",
month = "7",
day = "1",
doi = "10.1016/j.jdent.2019.05.023",
language = "English",
volume = "86",
pages = "33--40",
journal = "Journal of Dentistry",
issn = "0300-5712",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - Survival of human dental pulp cells after 4-week culture in human tooth model

AU - Pedano, Mariano S.

AU - Li, Xin

AU - Jeanneau, Charlotte

AU - Ghosh, Manosij

AU - Yoshihara, Kumiko

AU - Van Landuyt, Kirsten

AU - About, Imad

AU - Van Meerbeek, Bart

PY - 2019/7/1

Y1 - 2019/7/1

N2 - Objectives: This study aimed to validate the human tooth model by investigating the growth efficiency, expression of mesenchymal stem cell (MSC) markers and differentiation ability of human dental pulp cells (hDPCs) harvested from extracted immature third molars and cultured for different periods. Moreover, the effect of exposure and capping with a hydraulic calcium-silicate cement on pulp tissue after 4-week culture in the tooth model was investigated. Methods: Primary hDPCs were collected from 18 molars from six individuals (15–19 years). One tooth of each patient was immediately cultured (control), while the other teeth were exposed to culture medium for 1, 2 or 4 weeks. After different culture periods, cells were harvested using the explant method, upon which cells were evaluated for cell-doubling time, colony-forming efficiency and expression of cell surface markers. The osteogenic, adipogenic and chondrogenic differentiation efficacy was also determined. Two teeth from three different patients (n = 6) were used for the pulp-capping assay. Three teeth were capped with ProRoot MTA (Dentsply Sirona), while three other exposed teeth remained uncapped (control). Results: Cells were found to grow, express MSC markers and showed osteogenic, adipogenic and chondrogenic differentiation potential at all time periods. Histology of the teeth subjected to the pulp-capping assay showed the formation of mineralized tissue after 4-week exposure to ProRoot MTA (Dentsply Sirona) and normal histological features in the control teeth. Conclusions: This study confirmed that hDPCs of teeth cultured for up to 4 weeks in a human tooth model are viable, express MSC markers and show differentiation ability. Clinical Significance: The human tooth model can be seen as an advanced cell-culture model that makes use of the original 3D pulp-chamber structure. It can serve as a screening tool to evaluate new pulp-capping formulations in a relatively cheap and fast manner.

AB - Objectives: This study aimed to validate the human tooth model by investigating the growth efficiency, expression of mesenchymal stem cell (MSC) markers and differentiation ability of human dental pulp cells (hDPCs) harvested from extracted immature third molars and cultured for different periods. Moreover, the effect of exposure and capping with a hydraulic calcium-silicate cement on pulp tissue after 4-week culture in the tooth model was investigated. Methods: Primary hDPCs were collected from 18 molars from six individuals (15–19 years). One tooth of each patient was immediately cultured (control), while the other teeth were exposed to culture medium for 1, 2 or 4 weeks. After different culture periods, cells were harvested using the explant method, upon which cells were evaluated for cell-doubling time, colony-forming efficiency and expression of cell surface markers. The osteogenic, adipogenic and chondrogenic differentiation efficacy was also determined. Two teeth from three different patients (n = 6) were used for the pulp-capping assay. Three teeth were capped with ProRoot MTA (Dentsply Sirona), while three other exposed teeth remained uncapped (control). Results: Cells were found to grow, express MSC markers and showed osteogenic, adipogenic and chondrogenic differentiation potential at all time periods. Histology of the teeth subjected to the pulp-capping assay showed the formation of mineralized tissue after 4-week exposure to ProRoot MTA (Dentsply Sirona) and normal histological features in the control teeth. Conclusions: This study confirmed that hDPCs of teeth cultured for up to 4 weeks in a human tooth model are viable, express MSC markers and show differentiation ability. Clinical Significance: The human tooth model can be seen as an advanced cell-culture model that makes use of the original 3D pulp-chamber structure. It can serve as a screening tool to evaluate new pulp-capping formulations in a relatively cheap and fast manner.

KW - Biomaterials

KW - Calcium-silicate cements

KW - Pulp biology

KW - Pulp capping

KW - Tissue engineering

KW - Vital pulp therapy

UR - http://www.scopus.com/inward/record.url?scp=85067394195&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85067394195&partnerID=8YFLogxK

U2 - 10.1016/j.jdent.2019.05.023

DO - 10.1016/j.jdent.2019.05.023

M3 - Article

VL - 86

SP - 33

EP - 40

JO - Journal of Dentistry

JF - Journal of Dentistry

SN - 0300-5712

ER -