Surfactant protein D regulates airway function and allergic inflammation through modulation of macrophage function

Katsuyuki Takeda, Nobuaki Miyahara, Yeong Ho Rha, Christian Taube, Eun Seok Yang, Anthony Joetham, Taku Kodama, Annette M. Balhorn, Azzeddine Dakhama, Catherine Duez, Amanda J. Evans, Dennis R. Voelker, Erwin W. Gelfand

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

The lung collectin surfactant protein D (SP-D) is an important component of the innate immune response but is also believed to play a role in other regulatory aspects of immune and inflammatory responses within the lung. The role of SP-D in the development of allergen-induced airway inflammation and hyperresponsiveness (AHR) is not well defined. SP-D levels progressively increased up to 48 hours after allergen challenge of sensitized mice and then subsequently decreased. The levels of SP-D paralleled the development of airway eosinophilia and AHR. To determine if this association was functionally relevant, mice were administered rat SP-D (rSP-D) intratracheally. When given to sensitized mice before challenge, AHR and eosinophilia were reduced by rSP-D in a dose-dependent manner but not by mutant rSP-D. rSP-D administration resulted in increased levels of interleukin (IL)-10, IL-12, and IFN-γ in bronchoalveolar lavage fluid and reduced goblet cell hyperplasia. Culture of alveolar macrophages together with SP-D and allergen resulted in increased production of IL-10, IL-12, and IFN-γ. These results indicate that SP-D can (negatively) regulate the development of AHR and airway inflammation after airway challenge of sensitized mice, at least in part, by modulating the function of alveolar macrophages.

Original languageEnglish
Pages (from-to)783-789
Number of pages7
JournalAmerican Journal of Respiratory and Critical Care Medicine
Volume168
Issue number7
DOIs
Publication statusPublished - Oct 1 2003
Externally publishedYes

Fingerprint

Pulmonary Surfactant-Associated Protein D
Macrophages
Inflammation
Allergens
Alveolar Macrophages
Eosinophilia
Interleukin-12
Interleukin-10
Pulmonary Surfactant-Associated Proteins
Collectins
Goblet Cells
Bronchoalveolar Lavage Fluid
Innate Immunity
Hyperplasia

Keywords

  • Airway hyperresponsiveness
  • Eosinophils
  • Macrophages
  • Surfactant protein D

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Surfactant protein D regulates airway function and allergic inflammation through modulation of macrophage function. / Takeda, Katsuyuki; Miyahara, Nobuaki; Rha, Yeong Ho; Taube, Christian; Yang, Eun Seok; Joetham, Anthony; Kodama, Taku; Balhorn, Annette M.; Dakhama, Azzeddine; Duez, Catherine; Evans, Amanda J.; Voelker, Dennis R.; Gelfand, Erwin W.

In: American Journal of Respiratory and Critical Care Medicine, Vol. 168, No. 7, 01.10.2003, p. 783-789.

Research output: Contribution to journalArticle

Takeda, K, Miyahara, N, Rha, YH, Taube, C, Yang, ES, Joetham, A, Kodama, T, Balhorn, AM, Dakhama, A, Duez, C, Evans, AJ, Voelker, DR & Gelfand, EW 2003, 'Surfactant protein D regulates airway function and allergic inflammation through modulation of macrophage function', American Journal of Respiratory and Critical Care Medicine, vol. 168, no. 7, pp. 783-789. https://doi.org/10.1164/rccm.200304-548OC
Takeda, Katsuyuki ; Miyahara, Nobuaki ; Rha, Yeong Ho ; Taube, Christian ; Yang, Eun Seok ; Joetham, Anthony ; Kodama, Taku ; Balhorn, Annette M. ; Dakhama, Azzeddine ; Duez, Catherine ; Evans, Amanda J. ; Voelker, Dennis R. ; Gelfand, Erwin W. / Surfactant protein D regulates airway function and allergic inflammation through modulation of macrophage function. In: American Journal of Respiratory and Critical Care Medicine. 2003 ; Vol. 168, No. 7. pp. 783-789.
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